抗氰烯菌酯的禾谷镰刀菌nit突变体的诱导及其生物学特性.pdfVIP

中国农业科学 2007,40(4):735-740 Scientia Agricultura Sinica 抗氰烯菌酯的禾谷镰刀菌 nit 突变体的诱导及其生物学特性 陈 雨，陈长军，王建新，金丽华，周明国 （南京农业大学植保学院农业部病虫监测与治理重点实验室，南京 210095 ） 摘要：【目的】研究抗氰烯菌酯的禾谷镰刀菌nit 突变体的生物学特性。【方法】将3个抗氰烯菌酯的禾谷镰 刀菌（Fusarium graminearum Schw.）菌株分别在含氯酸盐的MMC培养基上培养，共获得了50个硝酸盐利用缺陷 突变体(nit)。比较了抗性菌株的 nit 突变体与亲本在无性和有性阶段的主要生物学性状。【结果】抗性菌株的nit 突变体与亲本在菌落生长速率、培养性状和致病性方面没有显著差异；但某些突变体中，产孢量和产子囊壳能力 方面存在一定差异。此外，禾谷镰刀菌对氯酸盐和氰烯菌酯之间没有交互抗药性，且抗性可以稳定遗传。【结论】 可以将 nit 作为遗传标记来研究禾谷镰刀菌对氰烯菌酯的抗药性遗传学。 关键词：禾谷镰刀菌；氰烯菌酯抗药性；生物学性状；遗传标记 Induction and Characterization of Nitrate Nonutilizing Mutants of Fusarium graminearum Resistant to Fungicide JS399-19 CHEN Yu, CHEN Chang-jun, WANG Jian-xin, JIN Li-hua, ZHOU Ming-guo (Lab of Monitoring and Management of Plant Diseases and Pests, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095) Abstract: 【Objective 】Nit mutants of Fusarium graminearum resistant to JS399-19 was characterized. 【Method 】Fifty nitrate nonutilizing mutants (nit) were obtained by transferring the 3 JS399-19-resistant mutants of Fusarium graminearum onto MMC media. 【Result 】Results showed that there were no significant differences in mycelial growth rate, cultural characters and pathogenicity between JS399-19-resistant nit mutants and their parental isolates. The conidiophore production and perithecigerous capacity changed more or less in some mutants. Results also indicated that there were no cross-resistance against chlorate and fungicide JS399-19 in Fusarium graminearum, and the resistance to both chlorate and JS399-19 could be transferred by asexual reproduction steadily. 【Conclusion 】Nit can be used as a genetic marker for genetic studies of Fusarium graminearum r