喹诺酮类药物抗体制备研究进展及策略分析.pdfVIP

中国农业科学 2008,41(10):3311-3317 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2008.10.052 喹诺酮类药物抗体制备研究进展及策略分析 王战辉，沈建忠，张素霞 （中国农业大学动物医学院/ 国家兽药残留基准实验室，北京 100193 ） 摘要：氟喹诺酮类（Fluoroquinolones，FQs）各单体药物之间的化学结构极其类似，通常针对某一个药物 制备的抗体对多种氟喹诺酮具有广谱识别性，是易于建立多残留免疫分析的代表性药物。本文综述了氟喹诺酮抗 体制备和食品中的免疫分析研究进展，分析了已发表文章中以不同氟喹诺酮为半抗原所获得抗体对其它氟喹诺酮 的交叉反应，并应用SYBYL.7.0程序包以MMFF94力场优化了8种批准用于动物的氟喹诺酮最低能量构象，从二维 和三维构象角度对制备广谱氟喹诺酮抗体的策略进行了探讨，理论上提出了制备广谱氟喹诺酮抗体的途径。 关键词：氟喹诺酮；广谱抗体；免疫分析；分子模拟 Recent Advances and New Strategy in Antibody Preparation for Fluoroquinolones WANG Zhan-hui, SHEN Jian-zhong, ZHANG Su-xia (College of Veterinary Medicine, China Agricultural University/National Reference Laboratory for Test of Veterinary Drug Residues, Beijing 100193) Abstract: The antibody against one of the fluoroquinolones (FQs) alsways possesses broad-spectrum binding characteristics to FQs due to the similarity between individual drugs of FQs, which are easy to be detected by multi-residue immunoassay. This paper reviewed advances in the development of immunoassay for FQs in food samples and analyzed the cross-reaction results from papers published, optimized the minimum energy conformations of 8 FQs approved to be used in animals. A theoretic strategy for obtaining broad-spectrum antibody was put forward based on the evidence of two-dimensional and three-dimensional view. Key words: Fluoroquinolone; Broad-spectrum antibody; Immunoassay; Molecular modeling 氧氟沙星（ofloxacin，OFL ）和马保沙星（marbofloxacin ， 0 引言 [5,6] （图2 ）。 MAR ） 氟喹诺酮（fluoroquinolones，