四川省葛藤属根瘤菌的遗传多样性研究.pdfVIP

中国农业科学 2004,37(11):1641-1646 Scientia Agricultura Sinica 四川省葛藤属根瘤菌的遗传多样性研究 1, 2 1 2 2 3 陈 强 陈文新 张小平 李登煜 K. Lindstrom 1 农业部农业微生物资源及其应用重点开放实验室/ 中国农业大学生物学院 北京100094 2 四川农业大学资源环境学院 雅安 625000 3Department of Applied Chemistry and Microbiology, University of Helsinki, Finland Fin-00014 摘要 用AFLPREP-PCR23S rDNA PCR-RFLP等方法对分离自四川的23株葛藤属的根瘤菌的遗传特性进行 了研究 结果表明 供试的葛藤根瘤菌遗传特性存在明显差异 AFLP分析结果显示 23个菌株分为7个AFLP遗 传群 在BOXAIR-PCR分析中 23株根瘤菌则被分为5个遗传群 对23S rDNA PCR-RFLP分析表明 葛藤根瘤菌 分属于中慢生根瘤菌属 Mesorhizobium慢生根瘤菌属 Bradyrhizobium2个属 聚类分析结果将其划分为5 个遗传群 分离自四川省的葛藤根瘤菌存在较大的遗传多样性 关键词 根瘤菌 遗传多样性 葛藤 AFLP Genetic Diversity of Rhizobia Isolated from Pueraria spp. in Sichuan , China 1, 2 1 2 2 3 CHEN Qiang , CHEN Wen-xin , ZHANG Xiao-ping , LI Deng-yu , K. Lindstrom 1 ( Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences China Agricultural University, Beijing 100094; 2College of Resources and Environmental Science, Sichuan Agricultural University, 3 Yaan 625000; Department of Applied Chemistry and Microbiology, University of Helsinki, Finland Fin -00014 ) Abstract: Twenty-three rhizobial strains isolated from Pueraria spp. in different sites of Sichuan province were studied by using AFLP, BOXAIR-PCR and 23S rDNA PCR-RFLP techniques. The results showed that genetic diversity among these rhizobia was existed. The analysis of AFLP revealed that, there were seven AFLP genotypes among these rhizobial strains ; and BOXAIR-PCR suggested that, there were five genoty