甜质型与普通型玉米籽粒发育过程中糖代谢相关酶活性的比较.pdfVIP

中国农业科学 2005,38(1):52-58 Sicientia Agricultura Sinica 甜质型与普通型玉米籽粒发育过程中糖代谢 相关酶活性的比较 刘 鹏，胡昌浩，董树亭，王空军，张吉旺，张保仁 （山东农业大学/山东省作物生物学重点实验室，泰安 271018） 摘要：对甜质型(Zea mays L. Seccharata Sturt)和普通型玉米（Zea mays L. Indentata Sturt ）籽粒发育过程中蔗 糖代谢和淀粉合成相关酶活性的变化动态进行了比较研究。结果表明，甜质型玉米籽粒发育过程中，蔗糖合成酶 （SS ）（包括合成与降解两个方向）和酸性蔗糖转化酶（Invertase ）活性高于普通型玉米。甜质型玉米籽粒腺苷二 磷酸葡萄糖焦磷酸化酶（ADPG-PPase ）、尿苷二磷酸葡萄糖焦磷酸化酶（UDPG-PPase ）、可溶性淀粉合成酶（SSS ） 活性高于普通型玉米，而束缚态淀粉合成酶（GBSS ）、淀粉分支酶（SBE ）和淀粉脱分支酶（SDE ）活性低于普 通型玉米，甜质型玉米SDE 活性低是大量积累水溶性多糖（WSP ）的直接原因，总淀粉积累量少和直链淀粉比例 小是GBSS 、SBE、SDE 综合作用的结果。 关键词：甜质型玉米；籽粒；蔗糖；淀粉；酶活性 Comparison of Enzymes Activity Associated with Sucrose Metabolism in the Developing Grains Between Sweet Corn and Normal Corn LIU Peng ，HU Chang-hao ，DONG Shu-ting ，WANG Kong-jun ，ZHANG Ji-wang ZHANG Bao-ren （Shandong Key Laboratory of Crop Biology /Shandong Agricultural University, Taian 271018 ） Abstract ：Field experiments were carried out to compare the dynamic variations of enzymes activities of sucrose and starch metabolism in the developing grains of sweet corn (Zea mays L. Seccharata Sturt) and normal corn (Zea mays L. Indentata Sturt). The activities of SS (syntheses and degradation direction) and invertase in grains of sweet corn were higher than those of normal corn, so the increase of sucrose in sweet corn is resulted from the increase of sucrose synthetic ability, but not the reduction of sucrose degradation. The activities of ADPG-PPase, UDPG-PPase and SSS were higher in sweet corn than normal corn, but that of the SSS, SBE, SDE were lower than normal corn. The reductions of SDE activity lead to the accumulation of WSP. The decreases of total starch and amylose percent in total st