npr1基因的隆及西瓜的遗传转化的研究.pdfVIP

NPR1 : : : NPR1 PCR NPR1 NPR1 ””, ,, NPR1 : DNA-PCR NPR1 NPR1 NPR1 pCaMVNPR , 35 BA , 0.2 1.0mg/L BA ,,,8-12 : :MS+BA1.0mg/L, 96%; :MS+BA0.2mg/L, 100%,MS IBA,NAA , 1/2MS+ NAA0.2+IBA0.05 ,,, :3d 1d, 10-15min,3d20mg/L MS 2~3 ,, 20mg/L 2~3 , MS+BA0.2+NAA0.05 1.52cm Hy ,5mg/l 200 DNA ,PCR 12 ,5 , I CLONING OF THE ARABIDOPSIS NPR1 GENE AND STUDY THE GENETIC TRANSFORMATION OF CITRULLUS VULGARIS Thesis author: Zhang Li Zhen Supervisor: Qin Xin min Abstract Experiments of obtaining more favorous watermelon were made with cultured Cotyledons of homozygous variety HeShan by combining plant tissue culture with Agrobacterium mediated transformation. In this research, we cloning of the Arabidopsis NPR1 gene, establishment of high efficiency regeneration system, determination of parameters involved in Agrobacterium mediated transformation Screening and characterization of transforming were systematically research .The results were summarized as follow: The NPRl gene was amplified from Arabidopsis thaliana genome DNA by DNA-PCR method. The DNA sequenced analysis showed that the sequence of amplified NPRl gene was the same as the published sequence. Thereforethe plant expression vector pCaMVNPR containing NPRl gene was constructed. The experiment will provide avenues for NPRl gene application in plant disease resistant genetic engineering. Success in establishment of high efficiency regeneration system was fundamental to the experiments. Many factors influenced re