agab-pedna病毒状颗粒的研究.pdf

Postdoctoral Fudan Thesis， University Abstract Gene 0n DNAvehicleisall than non—viral attractive viral delivery—based approach vector。Severalnon-viral have DNAtransfer been synthetic systems developed芏o as overcome wellintracellular rive transfer barriers．However,in physiological，as gene witll to non-viralhavebeenratherlowin viral efficacies vectors vectors．The comparison idealnon-viralvectorfor should DNAintoneutral deliverycondense gene complexes， avoid interactions、Ⅳitllcells．mediatecell andavoidDNA specific toxicity binding inacidic ofnon-virusvectorsintocellsis degradationvesicles。Targeteddelivery likely to the hasbeenusedto delivery improve efficiency．Galactosylatedpolyethyleneimine increasethetransfectionofDNAin cells．And efficiency hepatoma antibody—mediated of and ofDNAhavebeen to lipid-mediated targetingparticles targeting exploited increasethe and transfeetion delivery gene