hif-1α与fr mrna在脑白质软化动物模型中的表达及相关性研究.pdf

万方数据 目 录 中文摘要 ····························································1 英文摘要 ····························································3 前 言 ······························································5 第一部分 脑室周围白质软化模型的建立 1 引言 ·························································7 2 材料与方法 ···················································7 3 结 果 ·······················································17 4 讨 论 ·······················································24 5 结 论 ·······················································28 第二部分 HIF-1 α与TfR mRNA 在脑白质软化动物模型中的表达及相关性 1 引言 ·························································29 2 材料与方法 ···················································30 3 结 果 ·······················································35 4 讨 论 ·······················································41 5 结 论 ·······················································43 参考文献 ··························································44 英文缩略词表 ······················································47 致 谢 ····························································48 综 述 ····························································49 万方数据 HIF-1α 与 TfR mRNA 在脑白质软化动物模型中的表达 及相关性研究 中文摘要 【目的】 建立新生 4 天 SD 大鼠脑室周围白质软化模型（periventricular lerkomalacia, PVL ）。在构建 PVL 模型大鼠的条件下，研究 HIF- 1 α对TfR mRNA 表达的调 节，为缺氧缺血脑病发生机制与脑铁代谢之间关系的研究提供实验基础依据。 【方法】 1． 4 日龄新生SD大鼠通过随机数字表随机分为两组：PVL组和对照组；PVL组 采用分离结扎双侧颈总动脉并予缺氧的方法建立脑室周围白质软化新生大 鼠动物模型；对照组仅分离双侧颈总动脉，不予结扎及缺氧。采用HE染色 法观察脑组织病理学改变，Western blot法检测MBP蛋白表达，实验动物生 长发育及神经