响应面法优化脱脂米糠膳食纤维提取工艺研究.pdfVIP

64 CH INA O IL S AND FA TS 2009 V o l34 N o6 苗 欣, 张 晖, 李 伦, 郭晓娜, 肖 伟 ( 江南大学食品学院, 江苏无锡 2 14122) : 为优化脱脂米糠膳食纤维 取工艺, 在单因素试验基础上, 选择 N aOH 浓度N aOH 浸泡时 间碱性蛋白酶添加量和高温淀粉酶添加量为自变量, 膳食纤维纯度为响应值, 利用 Box - Benhnken 中心组合试验和响应面分析法, 研究各自变量交互作用及其对膳食纤维纯度的影响, 模 拟得到二次多项式回归方程的预测模型, 并确定脱脂米糠膳食纤维 取工艺为: N aOH 浓度 0. 2 m o l/L, N aOH 浸泡时间 60 m n, 碱性蛋白酶添加量 250 U / g, 高温淀粉酶添加量 200 U / g, 在此条件 下膳食纤维的纯度达到 75. 79% , 得率为 385% : 脱脂米糠; 膳食纤维; 响应面法 : T S229; R 151 : A : 1003- 7969 ( 2009) 06- 0064- 05 Optim ization of dietary fibre extraction from defatted rice bran by response surface m ethodology M IAO X n, ZHANG H u , L I Lun, GUO X aona, X IAO W e ( School of F ood Sc ence and T echno logy, J angnan Un vers ty, W ux 2 14122, J angsu, Ch na) Abstract: T he optm um cond t ons of ex tract ng d etary f bre from defa tted r ce bran w ere determ ned by response surface m ethodo logy. On the bas s o f s ngle facto r test, the m athem at ca l reg ress on m odelw as estab l shed about the dependent var able ( pur ty of d etary f bre) and ndependent var ab les ( concentra t on of N aOH, soak ng t m e of N aOH, dosage o f A lca lase and dosage of h gh temperature Am y lase ) through Box- Benhnken center com pos te des gn and response surface m ethodology. T he opt mum extrac t on cond t ons w ere as fo llow s: concentrat on o f N aOH 0. 2 m ol/L, soak ng tm e of N aOH 60 m n, dosage of A lca lase 250 U / g, dosage of h gh tem perature Am y lase 200 U / g. U nder these cond t ons, the pur ty o f d etary f bre w as 75. 79% , and the y e ld w as 38. 5% . K ey words: defatted r ce bran; d etary f bre; response surface m ethodo logy