结缔组织生长因子在肺纤维化初期肺血管表达.pdfVIP

生理学报 Acta Physiologica Sinica, August 25, 2008, 60 (4): 535-540 535 研究论文 结缔组织生长因子在肺纤维化初期肺动脉中的表达 * 崔茂香，陈晓玲 ，霍春玲，胡晓杰，艾洁 河北医科大学基础医学研究所病理生理学研究室，石家庄 050017 摘 要：本研究观察了博莱霉素(bleomycin, BLM)诱导肺纤维化初期肺动脉压、肺动脉壁I 、III 型胶原的含量以及肺动脉 壁结缔组织生长因子(connective tissue growth factor, CTGF)免疫阳性表达和分布。用气管内一次性滴注BLM (5 mg /kg 体重) 的方法复制肺纤维化动物模型大鼠；用右心漂浮导管技术检测肺动脉压；用天狼星红胶原纤维特异染色和偏振光观察肺动 脉I 、III 型胶原；用免疫组织化学法检测肺动脉壁CTGF 表达。结果显示：滴注BLM 后第14 天，大鼠肺动脉压高于对 照组大鼠(P0.05) ；肺动脉主干和肺内动脉壁I 、III 型胶原的染色面积大于对照组大鼠(P 0.05 ，P0.01) ，肺动脉主干血 管壁I 、III 型胶原染色面积的比值高于对照组大鼠(P0.05)；肺动脉主干和肺内动脉壁CTGF 免疫染色面积均大于对照组大 鼠，平均光密度也高于对照组大鼠(均P0.05)；增多的CTGF 免疫阳性细胞主要分布在肺动脉的平滑肌层和内皮层。以上 结果表明，在BLM 致肺纤维化形成初期肺动脉高压和肺血管壁结构重塑过程中，肺动脉壁平滑肌层和内皮层CTGF 表达 增多，这可能是肺动脉高压维持和发展的机制之一。 关键词：结缔组织生长因子；重塑；肺动脉；博莱霉素；纤维化；肺性；大鼠 中图分类号：R 3 6 3 Expression of connective tissue growth factor in pulmonary artery at the early- stage of pulmonary fibrosis * CUI Mao-Xiang, CHEN Xiao-Ling , HUO Cun-Ling, HU Xiao-Jie, Ai Jie Department of Pathophysiology, Ins titute of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 050017, China Abstract: To ascertain whether connective tissue growth factor (CTGF) participates in the remodeling of pulmonary artery at the early- stage of bleomycin (BLM)-induced pulmonary fibrosis, mean pulmonary arterial pressure, the expression of type I and type III collagens, and the expression and location of CTGF in pulmonary artery and arteriole were investigated in the present study. Sprague-Dawley rats received instillation of BLM [5 mg/kg body weight, in 0.5 mL of normal saline (NS)] or instillation of the same amount of NS as control. Mean pulmonary arterial pressure was detected via a catheter in the pulmonary artery. Type I and type III collagens were examined with Sirius red staining under polarized light. CTGF expression was investigated by using immunohistochemistry, and was represented as ave