TheInductionofCytotoxicTCellsandTumorRegressionbySoluble.pdfVIP

[CANCER RESEARCH 55, 3486-3489. August 15. 1995] Advances in Brief The Induction of Cytotoxic T Cells and Tumor Regression by Soluble Antigen Formulation Kandasamy Mariliaran, Gary Braslawsky, Amelia Black, Syamal Raychaudhuri,2 and Nabil Hanna 1DEC Pharmaceuticals Corporation. San Diego, California 92121 Abstract proteins and peptides conjugated to lipophilic compounds or formu lated with immune stimulating complexes or pH-sensitive liposomes CTLs specific for tumor antigens play a major role in the immunity have been used to elicit antigen-specific CD8+ CTL response against cancer. We have shown that class I-restricted CTLs can be in (10-13). duced by injecting soluble antigens mixed in an antigen formulation (AF) Recently, we have described a chemically defined AF3 composed that consists of squalane, Tween 80, and Pluronic L121 (S. Raychaudhuri et al., Proc. Nati. Acad. Sci. USA, 89: 8308-8312, 1992). In this study, of squalane, Tween 80, and Pluronic LI 21 and demonstrated that soluble antigens injected with AF induce antigen-specific CD8+ class using ovalbumin and the ovalbumin-expressing transfectoma (EG7) as a tumor model