三种水生植物内生真菌多样性和其抗真菌活性.pdfVIP

微生物学通报 SEP 20, 2009, 36(9): 1305~1310 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 三种水生植物内生真菌多样性及其抗真菌活性 赵春安 罗珊珊 毕艳艳 李海燕* (昆明理工大学生命科学与技术学院 云南 昆明 650224) 摘 要: 从华凤仙(Impatiens chinensis L.) 、问荆(Equisetum arvense L.)和轮叶狐尾藻(Myriophyllum verticillatum L.) 3 种植物中共分离到内生真菌 155 株。经鉴定, 它们分属于26 个不同的分类单元。 以杨桃炭疽菌(Colletotrichum gloeosporioides) 、香蕉疫霉(Phytophthora nicotianae)和腐皮镰刀菌 (Fusarium solani)等6 种植物病原真菌为指示菌, 对3 种水生植物内生真菌代谢产物进行抗菌活性 研究。结果表明, 有 37 株内生真菌( 占分离菌株总数的23.9%)显示出对一种或多种病原菌的抑菌 活性。来自华凤仙、问荆和轮叶狐尾藻的抗菌活性菌株比例分别为29.2% 、13.9%及37.1%, 都远 低于已报道的陆生植物内生真菌中活性菌株的比例。抗菌活性菌株主要分布于枝孢属 (Cladosporium) 、木霉属(Trichoderma)和地霉属(Geotrichum)等5 个属中。 关键词: 水生植物, 内生真菌, 枝孢属, 抑菌活性 Diversity of Endophytic Fungi from Three Aquatic Plants and Their Antagonistic Activities in vitro * ZHAO Chun-An LUO Shan-Shan BI Yan-Yan LI Hai-Yan (Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650224, China) Abstract: 155 endophytic fungi were isolated from Equisetum arvense L., Impatiens chinensis L. and Myriophyllum verticillatum L. They were identified to 26 taxa. The antagonistic activities of these isolates against 6 isolates of plant pathogenic fungi were tested on the medium, and 37(23.9%) isolates were activ- itive against one or more phytopathogens. The percentage of antifungal active isolates from E. arvense L., I.