a novel gαs-binding protein, gas-2 like 2, facilitates the signaling of the a2a adenosine receptor：一种新的gαs-结合蛋白，有利于gas-2像2，腺苷a2a受体的信号转导.pdfVIP

Biochimica et Biophysica Acta 1833 (2013) 3145–3154 Contents lists available at ScienceDirect Biochimica et Biophysica Acta jour nal homepage: www.e lsevie r. com/ locate/ bbamcr A novel Gαs-binding protein, Gas-2 like 2, facilitates the signaling of the A2A adenosine receptor Yi-Chih Wu a,b,c, Hsing-Lin Lai c, Wei-Cheng Chang c, Jiun-Tsai Lin c, Yu-Ju Liu d, Yijuang Chern b,c,d,⁎ a Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan b Taiwan International Graduate Program in Molecular Medicine, National Yang-Ming University and Academia Sinica, Taipei, Taiwan c Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan d Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan a r t i c l e i n f o a b s t r a c t Article history: The A2A adenosine receptor (A2AR) is a G-protein-coupled receptor that contains a long cytoplasmic carboxyl Received 21 June 2013 terminus (A2AR-C). We report here that Gas-2 like 2 (G2L2) is a new interacting partner of A2AR-C. The interac- Received in revised form 8 August 2013 tion between A2AR and G2L2 was verified by GST pull-down, co-immunoprecipitation, immunocytochemical Accepted 12 August 2013 staining, and fluorescence resonance energy transfer. Expression of G2L2 increased the intracellular cAMP con- Available online 27 August 2013 tent evoked by A2AR in an A2AR-C-dependent manner. Immunoprecipitation and pull-down assays demonstrated