a novel method for detecting amino acids derivatized with phenyl isothiocyanate by high-performance liquid chromatography–electrospray ionization mass spectrometry：一种检测苯基异硫氰酸酯衍生氨基酸的高效液相色谱法–电喷雾电离质谱新方法.pdfVIP

G Model MASPEC-15472; No. of Pages 6 ARTICLE IN PRESS International Journal of Mass Spectrometry xxx (2015) xxx–xxx Contents lists available at ScienceDirect International Journal of Mass Spectrometry j o u r n a l h o m e p ag e: w w w. elsev /locate/ijms A novel method for detecting amino acids derivatized with phenyl isothiocyanate by high-performance liquid chromatography–electrospray ionization mass spectrometry Guihua Zheng a , Wenwen Jin b , Pei Fan b , Xiaonan Feng a , Yun Bai b , Tao Tao a,∗∗ , Longjiang Yu b,∗ a School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China b Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China a r t i c l e i n f o a b s t r a c t Article history: High-performance liquid chromatography–electrospray ionization mass spectrometry (HPLC–ESI-MS) is Received 25 March 2015 an important technique for amino acid analysis. As a common derivatization reagent, phenyl isothio- Received in revised form 5 August 2015 cyanate (PITC) is widely used in this field. However, researchers have long faced the critical problem of Accepted 7 August 2015 coupling efficient separation with simultaneous, sensitive MS detection. In this work, a novel HPLC–ESI- Available online xxx MS method based on PITC derivatization was established for de