a novel recombinant bivalent outer membrane protein of vibrio vulnificus and aeromonas hydrophila as a vaccine antigen of american eel (anguilla rostrata):一种新型双价重组外膜蛋白创伤弧菌和嗜水气单胞菌作为一种美洲鳗(安圭拉)疫苗抗原.pdfVIP

a novel recombinant bivalent outer membrane protein of vibrio vulnificus and aeromonas hydrophila as a vaccine antigen of american eel (anguilla rostrata):一种新型双价重组外膜蛋白创伤弧菌和嗜水气单胞菌作为一种美洲鳗(安圭拉)疫苗抗原.pdf

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a novel recombinant bivalent outer membrane protein of vibrio vulnificus and aeromonas hydrophila as a vaccine antigen of american eel (anguilla rostrata):一种新型双价重组外膜蛋白创伤弧菌和嗜水气单胞菌作为一种美洲鳗(安圭拉)疫苗抗原

Fish Shellfish Immunology 43 (2015) 477e484 Contents lists available at ScienceDirect Fish Shellfish Immunology journal homepage: www.elsevier.c om/locate/fsi Full length article A novel recombinant bivalent outer membrane protein of Vibrio vulnifi cus and Aeromonas hydrophila as a vaccine antigen of American eel (Anguilla rostrata ) * Guo SongLin , Lu PanPan, Feng JianJun, Zhao JinPing, Lin Peng, Duan LiHua Fishery College of Jimei University/Engineering Research Center of Modern Eel Industrial Technology of the Ministry of Education, PRC. Jimei University, Xiamen, Fujian 361021, China a r t i c l e i n f o a b s t r a c t Article history: The immogenicity of a novel vaccine antigen was evaluated after immunized American eels (Anguilla Received 24 September 2014 rostrata) with a recombinant bivalent expressed outer membrane protein (OMP) of Vibrio vulnifi cus and Received in revised form Aeromonas hydrophila . Three groups of eels were intraperitoneal (i.p) injected with phosphate-buffered 8 January 2015 saline (PBS group), formaline-killed-whole-cell (FKC) of A. hydrophila a nd V. vulnifi cus (FKC group) or the Accepted 22 January 2015 bivalent OMP (OMP group). On 14, 21, 28 and 42 days post-vaccination respectively, proliferation of the Available online 2 February 2015 whole blood cells, titers of specific antibody and lysozyme activities of experimental eels were detected.

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