a procoagulant chemically sulfated mannan：促凝化学酯化甘露聚糖.pdfVIP

Carbohydrate Polymers 136 (2016) 177–186 Contents lists available at ScienceDirect Carbohydrate Polymers j o u r na l h o me p a ge : w w w. elsev /locate/car bpol A procoagulant chemically sulfated mannan Ana Helena P. Gracher, Aline G. Santana, Thales R. Cipriani, Marcello Iacomini ∗ Laboratório de Química de Carboidratos, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná CEP 81531-980, Brazil a r t i c l e i n f o a b s t r a c t Article history: Disorders of hemostasis can produce innumerous problems. Polysaccharides have been studied both Received 10 November 2014 as anticoagulant and as procoagulant agents. A mannan with a main chain of -(1 → 6)-linked-Manp Received in revised form 1 September 2015 units, branched at O-2 mainly by side-chains of 2-O-linked--Manp units was chemically sulfated, struc- Accepted 8 September 2015 turally characterized by NMR and GC–MS (methylation, desulfation and methylation with trideuterated Available online 10 September 2015 iodomethane), and tested in vitro and in vivo on blood coagulation models. Chemical analyses indicate a high degree of substitution on the sulfated polysaccharide. This polymer acted as a procoagulant agent, Keywords: increasing blood coagulation in normal and hemophilic plasma, activated platelet aggregation and also Chemically sulfated polysaccharide