a simple polymerase chain reaction technique to detect and differentiate shigella and enteroinvasive escherichia coli in human feces：一个简单的聚合酶链反应技术检测和粪便中的痢疾和肠侵袭性大肠杆菌分化.pdfVIP

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a simple polymerase chain reaction technique to detect and differentiate shigella and enteroinvasive escherichia coli in human feces：一个简单的聚合酶链反应技术检测和粪便中的痢疾和肠侵袭性大肠杆菌分化.pdf

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a simple polymerase chain reaction technique to detect and differentiate shigella and enteroinvasive escherichia coli in human feces：一个简单的聚合酶链反应技术检测和粪便中的痢疾和肠侵袭性大肠杆菌分化

ELSEVIER A Simple Polymerase Chain Reaction Technique to Detect and Differentiate ShigeEZa and Enteroinvasive Escherichia coli in Human Feces Huo-Shu H. Houng, Orntipa Sethabutr, and Peter Echeverria A simple polymerasechain reaction (PCR) procedure using with low bacterial concentration; i.e., lo* CFLl/gram. Sero- IS630-specific primers was developed as a general diagnostic type-specific primers derived from the rfc genes ofdifferent probe to detect Shigella and enteroinvasive Escherichia coli Shigella strains were used in PCR reactions to differentiate (EIEC). However, IS630 and the other two previously reported among Shigella serotypes in the laboratory, such as S. sonnei, molecular probes, ipti and ial, cannot be used to differentiate S. flexneri, and S. dysenteriae 2. It was demonstrated fur- among Shigella serotypes and EIEC strains that cause dysen- ther that the multiplex PCR system containing rfc-specific tery. The sensitivity ofPCR protocol was determined to be primers can efficiently identify the most prominent Shigella ZOO-200 shigellae for each PCR reaction. An enrichment incu- serotypes in raw stool samples of acute diarrhea1 patients. bation would allow the detection of shigellae in stool samples 0 1997 Elsevier Science Inc. INTRODUCTION ronmental sources of these pathogens is unknown.