a simple system for converting lacz to gfp reporter fusions in diverse bacteria：将lacz基因gfp融合在不同的细菌，一个简单的系统.pdfVIP

Gene 372 (2006) 219 – 226 /locate/gene A simple system for converting lacZ to gfp reporter fusions in diverse bacteria Mark Goulian a,b,⁎, Marjan van der Woude c,⁎ a Department of Physics, University of Pennsylvania, Philadelphia, PA 19104, USA b Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA c Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104, USA Received 2 June 2005; received in revised form 3 January 2006; accepted 4 January 2006 Available online 24 February 2006 Received by D.L. Court Abstract We describe new plasmids that facilitate the rapid conversion of lacZ fusions to gfp transcriptional fusions in bacteria. The exchange is based on a double recombination between lacZ sequences in a suicide vector and the recipient chromosome. The suicide vector is a mobilizable, conditionally replicative plasmid that contains the gene for gfp with flanking lacZ homology and is derived from a broad host range plasmid that has been successfully used in a wide range of bacterial species. The technique was used to convert lacZ reporter fusions to gfp fusions in Escherichia coli, Bordetella bronchiseptica and Agrobacterium tumefaciens . Green fluorescent protein expression in the new recombinants reflected the beta galactosidase expression