a rabbit model to tissue engineer the bladder：一种组织工程膀胱的兔子模型.pdfVIP

ARTICLE IN PRESS Biomaterials 25 (2004) 1657–1661 A rabbit model to tissue engineer the bladder J.E. Nuiningaa ,H. van Moerkerkb ,A. Hanssenc ,C.A. Hulsbergend ,J. Oosterwijk-Wakkae E. Oosterwijke ,R.P.E. de Giera ,J.A. Schalkene ,T.H. van Kuppeveltb ,W.F.J. Feitza * a Department of Urology, Pediatric Urology Center, University Medical Center, Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands b Department of Biochemistry, University Medical Center, Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands c Animal Laboratory, University Medical Center, Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands d Department of Pathology, University Medical Center, Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands e Department of Experimental Urology, University Medical Center, Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands Abstract A rabbit model was used for the evaluation of a collagen-based biomatrix of small intestinal submucosa (SIS ,COOKs) in comparison to a biochemically reconstructed biomatrix for bladder tissue regeneration. Rabbits underwent partial cystectomy and cystoplasty with SIS patch graft or with a biochemically defined collagen biomatrix. The grafts of the regenerated bladder wall were harvested at different intervals and tissue regeneration was evaluated. The results of the SIS and biochemically defined biomatrix grafts were comparable. At harvesting ,we found five bladder stones and encrustation of the biomatrix in 21/56 animals. No stone formation was observed in the control group. The results of the molecularly defined biomatrix are thus far comparable to SIS. Both matrices show good epi