a simplified enzymatic method for total cholesterol determination in milk：牛奶中总胆固醇含量测定的简化方法.pdfVIP

下载本文档

5
0
约 8页
2016-03-12 发布于广东
举报
版权申诉

a simplified enzymatic method for total cholesterol determination in milk：牛奶中总胆固醇含量测定的简化方法.pdf

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

a simplified enzymatic method for total cholesterol determination in milk：牛奶中总胆固醇含量测定的简化方法

International Dairy Journal 50 (2015) 50e57 Contents lists available at ScienceDirect International Dairy Journal journal homepage: /locate/idairyj A simpliﬁed enzymatic method for total cholesterol determination in milk * Anna Malwina Kamelska , Beata Jarmołowska, Krzysztof Bryl University of Warmia and Mazury in Olsztyn, Faculty of Food Sciences, Poland a r t i c l e i n f o a b s t r a c t Article history: Methods for determination of milk cholesterol concentration were compared, with particular emphasis Received 25 March 2015 on an enzymatic method (ME). Bovine and human milk samples (n ¼ 40) were pre-treated and analysed Received in revised form by three different protocols: (i) IDF Standard with gas chromatography (GC); (ii) direct saponiﬁcation 16 June 2015 (DS) and GC; (iii) DS and ME with spectrophotometric determination. A simpliﬁed method (DS þ ME) Accepted 22 June 2015 was developed with an acceptable coefﬁcient of variation. The cholesterol concentration values assessed Available online 6 July 2015 by DS þ ME were higher compared with DS þ GC. There were no statistically signiﬁcant differences between the results obtained by the enzymatic and IDF method (P ¼ 0.09). The mean cholesterol con- 1 1