aberrant methylation of tumor suppressor genes in patients with refractory anemia with ring sideroblasts：伴环形铁粒幼细胞性贫血患者的抑癌基因的异常甲基化.pdf

Leukemia Research 35 (2011) 479–483 Contents lists available at ScienceDirect Leukemia Research j o u rn al h omepage: w w w. elsev /locate/leu kres Aberrant methylation of tumor suppressor genes in patients with refractory anemia with ring sideroblasts Ana Valencia a,∗ a a ˜ a a a , José Cervera , Esperanza Such , Mariam Ibanez , Inés Gómez , Irene Luna , Leonor Senent a , Silvestre Oltra b , Miguel A. Sanz a , Guillermo F. Sanz a a Department of Hematology, Hospital Universitario La Fe, Avda. Campanar 21, 46009 Valencia, Spain b Genetic Unit, Hospital Universitario La Fe, Valencia, Spain a r t i c l e i n f o a b s t r a c t Article history: This study evaluates the incidence and prognostic impact of aberrant methylation of 25 tumor suppressor Received 28 May 2010 genes in 40 patients with RARS, a MDS subtype, by methylation-specific multiplex ligation-dependent Received in revised form 29 July 2010 probe amplification (MS-MLPA) assay. Methylation of at least one gene was detected in 18 patients (45%). Accepted 22 August 2010 The genes methylated were CDKN2B (20%), RASSF1 (18%), RARB (10%), CDH13 (7.5%) and FHIT (5%). Patients Available online 22 September 2010 with at least one methylated gene had a significantly shorter OS than patients without methylated genes.