孕激素对子宫内癌ishikawa细胞tgf-β1、tβr1表达的影响.pdfVIP

中文论著摘要·····················································1 英文论著摘要····································一”··一·一······3 二、英文缩略词·······················································5 三、论文 ‘。。。‘。。‘。。’’。。。。‘。。。。。。。。。’。。。。。。。’。。。。。。。。。。‘。。。。。。。‘。。。‘。。‘ 自订一言·····························································1—5 日U吾 材料与方法·······················································7 实验结果························································10 讨论····························································14 结论····························································15 四、本研究创新性的自我评价··········································16 五、参考文献························································17 六、附录 综述····························································19 在学期间科研成绩················································28 致谢”··························································29 个人简介··················································一····30 ·中文论著摘要· shi 1、T13 孕激素对子宫内膜癌I kawa细胞TGF-13 R1表达的影响 目 的 本课题以高分化子宫内膜癌Ishikawa细胞株为研究对象，通过对孕酮(MPA) 作用后子宫内膜癌Ishikawa细胞的增殖、凋亡的检测以及TGF-131及其受体的表达 1、 情况的测定，观察MPA对人子宫内膜高分化腺癌细胞株Ishikawa细胞中TGF一6 T 13 BR1蛋白表达的影响，探讨孕酮作用与Ishikawa细胞TGF-Bl、TR1蛋白表达及 细胞凋亡之间的关系，为孕激素治疗子宫内膜癌的效应途径提供一定理论依据， 并且为子宫内膜癌的孕激素治疗提供新的思路。 方法 常规方法体外培养人子宫内膜高分化腺癌细胞株Ishikawa细胞，细胞随机分 u la la la 组，实验组分别以含不同浓度MPA(2．5g／ml、5g／ml、10g／m1、15g／ml、 20la la la 细胞在MPA作用不同时间梯度(24h、48h及72h)及浓度梯度(2．5g／m1、 g／ml、5 10 la p u g／ml、15g／ml、20