小干扰rna靶egfr基因逆转卵巢癌细胞顺铂耐药的研究.pdf

IIlr l l I 1111 Illll lll lll Y1 734319 RNA The ofEGFRGene InterferenceonReversal Study Silencingby Effectof to SKOV3／DDPCellCisplatin—Resistance Abstract To theeffectofvector-basedsmall the objectiveexplore InterferingRNA(siRNA)on ofEGFRanditsrolesin the resistanceonovariancancer expression reversingcisplatin cellSKOV3／DDP Methods toEGFR and shRNA mRNA According sequence，wedesignedsynthesized EGFRwhichwere clonedinto 2．1一U6neo targeted directly pSilencerrM plasmid．DNA confirmedwhethertheinsertion Wascorrectornot．Thentransfected sequencing sequence therecombinantintohumanovariancancercelllineSKOV3／DDP plasmid byliposome． The includedthenormalcontrolcell transfectant line，the study emptyplasmid group， and transfectant and were nonspecificspecific groups．RT-PCR to EGFRmRNAandits half detectthelevelof performedrespectively protein．The ineach Wasmeasured inhibitoryconcentration(IC50)ofgroup bymethyl cisplatin andthe ofthecellto Was thia