survivi基因在ⅱ期子宫颈癌的表达与子宫动脉灌注化疗关系研究.pdfVIP

摘 要 Survivin 基因在Ⅱ期子宫颈癌中的表达及与子宫动脉 灌注化疗关系研究 研究生：刘 静 导师：程静新 副教授 摘 要 目的：探讨Survivin基因在Ⅱ期子宫颈癌的表达及与子宫动脉灌注化疗的关系。 方法：收集了我院2004年1月-2007年12月期间收治的Ⅱ期宫颈癌患者77例，子宫动脉 灌注化疗前后标本154份。采用免疫组化法(PV-9000)检测Survivin基因的表达。结果： Survivin基因在Ⅱ期宫颈癌的总的阳性表达率为66.23 ﹪。 Survivin基因的表达与淋巴 结有无转移有关（P0.05 ），Survivin基因的表达与患者年龄、族别、组织学类型、 组织学分级、宫颈浸润深度无关，差异无统计学意义（P0.05 ）。77例Ⅱ期宫颈癌患 者经子宫动脉灌注化疗后与化疗前比较Survivin基因阳性表达下降，差异有统计学意 义（P0.05 ）。结论：子宫动脉灌注化疗可能通过下调了Survivin基因的表达达到了 治疗宫颈癌的目的。 关键词： 宫颈癌；子宫动脉灌注化疗；survivin 基因 —1— 新疆医科大学医学硕士学位论文 Survivin Expression of Stage ⅡCervical Carcinoma and relationship of Intra-uterine infusion Arterial Chemotherapy Postgraduate:liu Jing Supervisor: Associate Prof. Cheng Jingxin Abstract Objective: To evaluate the expression of survivin gene in cervical cancer tissue in stage Ⅱand relationship of Intra-uterine infusion arterial chemotherapy. Methods: seventy-se patients in uterine cervix cancer from January 2004 to December 2007 were enrolled in the study. The expression of survivin was checked by immunochemistry PV-9000 method. Results: The total express ratio of survivin was 66.23% in stage Ⅱcervical carcinoma. Express of survivin was relationship with lymph node status (P 0.05); but there was no relationship with age ,race, pathological types ,pathological grade ( P 0.05). Survivin expression was significant before and after chemotherapy in 77 cases of cervical carcinoma (P0.05). Conclusion: To treat cervical cancer intra-uterine arterial infusion chemotherapy may down- regulate expression of survivin. Key words : Cervical cancer; Intra-uterine infusion arterial chemotherapy; survivin gene