丝状真菌目标基因替换过程中的策略和方法.pdfVIP

HEREDITAS (Beijing) 2007 7 , 29(7): 898904 ISSN 0253-9772 DOI: 10.1360/yc-007-0898 1, 2, 1, 1, 1, 2 1. , 310021; 2. , 310029 : , , , : ; ; Strategies of targeted gene replacement in filamentous fungi 1, 2 1 1 1 2 WANG Jiao-Yu , DU Xin-Fa , CHAI Rong-Yao , SUN Guo-Chang , LIN Fu-Cheng 1. Institute of Plant Protection and Microbiology , Zhejiang Academy of Agricultural Science, Hangzhou 310021, China; 2. Colledage of Agriculture and Biotechnology, Zhejiang Universtiy, Hangzhou 310029, China Abstract: Targeted gene replacement (TGR) is an important technique for gene function analysis. With the development of genome sequencing and transformation, TGR has been applied widely to filamentous fungi, and many new systems and approaches have been established. In this paper, strategies involved in TGR in filamentous fungi were reviewed, including transformation systems, targeting vector construction, mutant selection and so on. Compared with TGR, RNAi and other techniques of gene function analysis were introduced and reviewed as well. Keywords: targeted gene replacement; filamentous fungi; gene function analysis (Targeted gene replacement, TGR) , , , , , , , 1 TGR TGR 20 80 DNA (homologous recombination, TGR HR), , [1] , TGR [2,3] CaCl /