大豆株高QTL的ldquo;整合rdquo;及其Overview分析.pdfVIP

HEREDITAS (Beijing) 2013 2 , 35(2): 215―224 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2013.00215 大豆株高QTL 的“整合”及Overview 分析 1,2 2 1 2 高利芳 , 郭勇 , 郝再彬 , 邱丽娟 1. , 541000; 2. , , 100081 株高是作物株型的重要组成因子, 与作物种植密度、抗倒伏性及产量等密切相关。文章利用Soybase 数 据库和文献报道的201 个与大豆株高相关的QTL 信息, 在物理整合的基础上, 采用软件BioMercator2.1 进行元 分析, 得到 15 个株高的“通用”QTL, 分别位于大豆的 6 号、7 号、11 号、13 号和 18 号染色体。同时, 利用基 于统计学原理的Overview 方法进行优化, 将这些QTL 位点的置信区间最小缩至0.1 cM, 从而明确了QTL 位点 在染色体上的遗传位置。对重演性较好的QTL 位点所对应的区段内的基因进行分析, 初步筛选出可能与株高相 关的候选基因 17 个。文章为大豆株高相关QTL 位点的精细定位及分子标记辅助育种奠定了基础。 大豆; 株高; 元分析; Overview 分析 Integration and “Overview” analysis of QTLs related to plant height in soybean 1,2 2 1 2 GAO Li-Fang , GUO Yong , HAO Zai-Bin , QIU Li-Juan 1. Guilin University of Technology, Guilin 541000, China; 2. The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/Institute of Crop Science , Chinese Academy of Agricultural Sciences , Beijing 100081, China Abstract: Plant height, one of the most important factors for plant architectures, is closely related with some important traits such as plant density, lodging resistance and yields.. In the present study, 201 QTLs related to plant height of soybean from database and references were analyzed. The results revealed that 15 consensus QTLs located on chromosomes 6, 7, 11, 13, and 18 were identified after physical integrations and Meta analysis using the software BioMercator 2.1. Moreover, “Overview” method was used to op