成年大脑神经细胞特异性ADAM10基因敲除小鼠模型的建立和鉴定.pdfVIP

HEREDITAS (Beijing) 2012 12 , 34(12): 1570―1576 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2012.01570 成年大脑神经细胞特异性ADAM10 基因敲除小鼠模型 的建立与鉴定 炤 刘军, 周常文, 韦秋兰, 庄建龙, 林 华, 郑杰辉 , , , 350004 10(ADAM10 ) 30 (sheddase), , , ADAM10 ADAM10 , ADAM10 CaMKIIα-Cre ADAM10 loxP/loxP , CaMKIIα-Cre/ADAM10 loxP/loxP , PCRADAM10 cKODNA, ADAM10 PCR, ADAM10 mRNA55.7%60.8% ; Western blotting, ADAM10 63%84.8% , ADAM10 cKO , ADAM10 , , ADAM10 (cKO), ADAM10 , ADAM10 ADAM10 ; ; ; Western blotting; Generation and characterization of the adult neuron-specific ADAM10 knock-out mice LIU Jun, ZHOU Chang-Wen, WEI Qiu-Lan, ZHUANG Jian-Long, LIN Zhao-Hua, ZHENG Jie-Hui Department of Cell Biology and Genetics, School of Basic Medicine / Center of Cell and Developmental Biology/ Provincial Key Labora- tory of Stem Cell and Regenerative Medicine, Fujian Medical University, Fuzhou 350004, China 收稿日期: 2012−03−26; 修回日期: 2012−05−03 基金项目: (2010j01171)(XZ04005) 作者简介: , , E-mail: 290178077@ 通讯作者: , , , E-mail: zhouchangwen624@ 致 谢: , 网络出版时间: 2012/8/9 10:45:07 URL: /kcms/detail/11.1913.R1045.002.html 12 : ADAM10 1571 Abstract: A disintegrin and metalloproteinase 10 (ADAM10) is a major sheddase fo