贞清方调节SREBP__1c减少糖尿病大鼠肾脏脂质沉积.docVIP

贞清方贞清方链脲佐菌素分：贞清方组、、模型组，另设正常组周检测尿微量白蛋白排泄率(UAE)肾脏行染色在光镜下作肾组织形态学观察P0.01）, 且SREBP-1c和FAS mRNA 的表达明显上调（P0.01）。贞清方（P0.01）贞清方（P0.01）P0.01）P0.05）贞清方（P0.0）UAE明显下降（P0.0P0.05）贞清方和胍贞清方贞清方WEN Xiu-ying, XU Ming-wang，HU Qin-jin,（ Liyuan Hospital , Tongji Medical College， Huazhong University of Science and Technology, Wuhan 430077, China） ABSTRACT: OBJECTIVE To investigate Zhenqing Recipe(ZQR) in regulation of renal lipid metabolism and its molecular mechanisms. Methods Type 2 diabetic rat model was established with feeding high-glucose and high-fat diet, and injecting low dose streptozotocin. The model rats were divided into: untreated model group, ZQR group, metformin group, normal rats served as control, 18 rats in each group. At week 8 and week 12 after treatment, TG contents in renal cortex were measured. And at week 12, the expression of sterol regulatory element binding-protein- 1c (SREBP-1c ) and fatty acid synthase (FAS) mRNA were detected by RT-PCR. Meanwhile, fasting blood glucose (FBG), total triglyceride (TG) and total cholesterol (TC) and urine albumin excretion (UAE) were measured. Kidney histomorphology was observed by the light microscope with HE staining. RESULTS At week 8 , the level of TG in renal cortex had a tendency to increase in model rats, however, there was no difference with control (P0.05). And the level of renal TG showed no obvious change in two treated groups. At week 12 , the renal TG was higher in untreated model rats compared with control(P0.01). In contrasted to control, expression of SREBP-1c and FAS mRNA in model group were up-regulated (P0.01). Contents of renal TG in ZQR and metformin groups were decreased compared with model group (P0.01). The expression of SREBP-1c was significantly down-regulated in ZQR group than model group (P0.01). And expression of SREBP-1c mRNA in metformin group was also decreased compared with model group (P0.01)，but it is higher than control(P0.05). Both treated groups showed lower level of FAS mRNA than th