甜菜内源激素和其抗丛根病的关系.pdfVIP

摘 要 植物内源激素参与植物体与病原菌的互作过程，已得到人们的广泛关注，本试验 分别在室内盆栽和田间自然栽培两种条件下，以甜菜抗、感丛根病品种为供试材料， 研究甜菜黄脉坏死病毒（BNYVV）侵染后内源激素的变化及其在抗、感丛根病品种间 的差异，以探究内源五大激素与甜菜抗丛根病的关系。研究结果表明，无论是在室内 盆栽或是田间自然栽培下，BNYVV侵染甜菜后，抗、感病品种的内源生长素（IAA）、 脱落酸（ABA）、乙烯（ETH）的含量较无病土（地）均有不同程度的提高，细胞分裂 素（CTK）、赤霉素（GA）的含量有所下降，通过与抗、感病品种病情指数的相关性 分析表明，内源IAA的增加量、iPA的相对含量（占无病地%）、GA的相对含量与病情 指数呈负相关，ABA、ETH的增加量与病情指数呈正相关；苗龄35天是BNYVV侵入较大 的时期，该时期可作为检测甜菜激素与抗病性关系的关键时期，IAA、iPA可作为甜菜 抗丛根病性鉴定和选育的生理指标。本研究从病毒侵染后内源激素的变化阐述了激素 在甜菜丛根病中的作用，为甜菜抗病机制以及选育抗病品种提供理论依据。 关键词: 甜菜；抗丛根病性；内源激素；甜菜黄脉坏死病毒 The Relationship between Endogenous Hormones and Rhizomania Resistance in Sugar beet (Beta vulgaris) Abstract Plant endogenous hormone is participate in the interaction process between plant and bacteria, it has received widely attention, this experiment under indoor and field cultivation conditions , using Rhizomania resistant and susceptible sugar beet varieties as experimental materials to study the variation of endogenous hormones after beet necrotic yellow vein virus (BNYVV) infection and differences between Rhizomania resistance and susceptible varieties, to explore the relationship of the five classes hormone and rhizomania resistance. The results show that both under the indo or cultivation and field conditions, after BNYVV infection, the endogenous Auxin (IAA), Abscisic Acid (ABA), Ethylene (ETH) levels of resistant and susceptible varieties in virus soil has different enhance than BNYVV free soil, Cytokinin (CTK), Gibberellin (GA) content is decrease, by the correlation analysis of disease index of the resistant and susceptible varieties ,the results show that the increase amount of endogenous IAA , iPA relative content (accounting for % in BNYVV free field),