一色齿毛菌对活性染料的脱色研究.pdfVIP

第36卷摇 第4期 北 京 林 业 大 学 学 报 Vol.36,No.4 2014年7月 JOURNAL OF BEIJING FORESTRY UNIVERSITY Jul. ,2014 DOI:10.13332/ ki.jbfu.2014.04.023 一色齿毛菌对活性染料的脱色研究 于摇 存摇 池玉杰 东北林业大学林学院 摘要:为进一步鉴定试验菌株及明确一色齿毛菌CB1 Cerena unicolor CB1 对活性染料的脱色效果,在对该菌株 ITS 序列克隆的基础上,进行了其对活性黑和活性红2 种活性染料脱色条件的研究。 结果表明,C. unicolor CB1与17 个同种其他菌株的ITS序列相似性为93% ~99%,说明试验菌株为一色齿毛菌。 染料脱色结果表明,250 mg/ L 的 活性黑对C. unicolor CB1的脱色产生明显的抑制,而500mg/ L 的活性红对该菌株的脱色抑制不明显。 C. unicolor 2+ 2+ CB1对活性黑和活性红的脱色最适碳源分别是果糖和葡萄糖;最适氮源分别是尿素和硝酸铵;最适Cu 和Mn 添 加浓度都为0郾1mmol/ L;最适pH为5;最适接种量为直径d 8mm 的新鲜菌丝7 片。 关键词:一色齿毛菌;ITS序列;活性染料;脱色 鄄鄄 鄄鄄 鄄鄄 中图分类号:X172摇 摇 文献标志码:A摇 摇 文章编号:1000 1522 2014 04 0126 07 YU Cun;CHIYu鄄jie. DecolorizationtotworeactivedyesofCerena unicolor CB1. Journalof Beijing 鄄鄄 Forestry University 2014 36 4 126 132 [Ch,24 ref.] College of Forestry, Northeast Forestry University,Harbin,150040,P.R.China. A study for determining the decolorizing capacity of white rot basidiomycete Cerena unicolor strain CB1was carried out. Based on cloning of ITS sequence,factors affecting decolorization into two dyes, reactiveblackandreactivered,werecarriedout. TheresultsofphylogeneticanalysisshowedthattheITS sequence of C. unicolor CB1was93% to99% homologousto 17 different C. unicolor strains,indicating that strain CB1 was C. unicolor. The results of decolorization tests showed that the reactive black dye inhibited the decolorization reaction of C. unicolor CB1 evidently at the concentration of 250 mg/ L, whereas reactive red had no significant inhibition to the decolorization reaction even at the concentration of 500mg/ L. The most optimal carbon sourcefor C. unicolor CB1to decolor reactive black and reactive red was fructose and glucose,respectively;The best nitrogen source was urea and ammonium nitrate, 2+ 2+ respectively. The optimal concentration of Cu and Mn both was0郾1mmol/ L,the optimum pH was 5,and the optimum inoculum was seven fresh mycelium discswith d 8mm. Key words摇 Cerena unicolor CB1;ITS sequences;reactive dyes;decolorization 鄄鄄 [3 4]