快律宁胶囊及其药血清对大鼠心室肌细胞内钙离子平均荧光强度的作用.pdfVIP

The Effect of KLN Capsule and its Drug Serum on Fluorescence Intensity of Intracellular Calcium in Rat Ventricular Muscle Cells Speciality：Internal Medicine of TCM Author ：PETRONIS EROTOKRITOU Tutor ：Professor Yang Chuan Hua Abstract Objective To observe the effect of KLN capsules (KLN) and its drug serum on mean fluorescence intensity of intracellular calcium in rat ventricular muscle cells. Methods SD rats were divided into four groups, control group and KLN high-dose group, KLN medium-dose group and KLN low-dose group. After intragastric administration, collect the blood to prepare KLN drug serum. Separate a single rat ventricular muscle cell. Using Fluo-3/AM probe to make the cells fluorescently labeled and observe [Ca2+] with a laser scanning confocal microscope. Add KLN or its drug serum i to extracellular solution between the 2nd and 3rd scan. Observe the effect of KLN on resting cells’ [Ca2+]i and release of intracellular calcium store, as well as the effect of KLN 2+ drug serum on the resting cells’ [Ca ] . i Results ① Compared with the control group, KLN high, medium and low dose could significantly increase [Ca2+] in resting cell cytoplasm (P0.05), showing a gradually i increased trend over time and dose dependence. ② In calcium-free Tyrodes solution, compared with the control group, KLN high, medium dose significantly increase [Ca2+] in