电针心俞、厥阴对心肌缺血再灌注大鼠心肌细胞凋亡及相关因素的影响.pdfVIP

The Study of Electro-Acupuncture Xinshu and Jueyinshu on Myocardial Apoptosis Related Factors in Ischemia Reperfusion Injury Specialtity：Acupuncture and Moxibustion Author ： Zhang Xin Tutor：Tan Qiwen Abstract Objective ： The aims of this study are to assess the protective effect of electro-acupuncture “Xinshu” and “Jueyinshu” on myocardial ischemia reperfusion injury in rats ，and to explore mechanism of anti-apoptosis related factors for acupuncture “Xinshu” and “Jueyinshu” on myocardial ischemia reperfusion injury. Methods ：All the wistar rats were randomly divided into 10 groups(n=8 in each group) ： (1)Sham-operation group Ⅰ、Ⅱ, (2)Model group Ⅰ、Ⅱ, (3)Treatment group Ⅰ、 Ⅱ, (4) Positive control group Ⅰ、Ⅱ, (5) Negative control group Ⅰ、Ⅱ. Series Ⅰwere treated for 3 days and series Ⅱ were treated for 7 days with electro-acupuncture. A comparative study on the apoptosis index(AI), were conducted The expression characteristics of target gene Fas/FasL, high temperature requirement A2(HtrA2), 5-lipoxygenase(5-LOX) and Cytochrome P450 monooxygenase （CYP450 ） in myocardial tissue were achieved. The apoptosis related factors and acupuncture relevance were analyzed. The plasma cytokines TNF-α and ICAM-1 were also measured by ELISA. Results ： 1. Compared with Sham-operation group, the expression of pro-inflammatory factors TNF-α and ICAM-1, the apoptosis index(AI),and the apoptosis related factors i.e. Fas ，FasL, HtrA2,CYP450 were significantly upregulated in Model group. 2. Compared with Model group, the apoptosis index, and the apoptosis related factors i.e. Fas ，FasL, HtrA2, CYP450 were significantly downre