糖网清汤对糖尿大鼠血流变及视网膜影响的实验研究.pdfVIP

中文摘要 目的：本实验采用链脲佐菌素（STZ）造成糖尿病大鼠模型，饲养3 个月，予以实验 性大鼠灌服中药复方“糖网清汤”，通过对糖尿病大鼠血液流变学、视网膜组织切片 及视网膜血管形态的改变，来初步探讨研究中药复方“糖网清汤”防治糖尿病视网膜 病变的部分作用机制。 方法：将链脲佐菌素（STZ）所致的糖尿病大鼠分成五组：糖尿病模型组（M）、多贝 斯组（A）、糖网清小剂量组（B）、糖网清中剂量组(C)、糖网清大剂量组(D) （n=10）， 并设置空白对照组（CON）。每日上午9:00 灌药一次，造模成功后每四周测一次随机 血糖，灌药3 个月后测血液流变学指标，并取大鼠眼球分别作视网膜铺片及视网膜组 织切片。 结果：糖网清组和多贝斯组血液流变学检测结果，两药相比，前者优于后者，且糖网 清大剂量组的疗效优于小剂量组。3 个月时糖尿病模型组大鼠的血液呈高凝、高粘现 象，并出现了小血管迂曲扩张及视网膜渗出等早期糖尿病视网膜病变的特征，而给予 中、西药的各组大鼠，视网膜血管的改变与模型组相比都有不同程度减轻，其中大剂 量组最接近空白组。 结论：糖尿病大鼠3 个月时，眼底和视网膜血管结构已初步发生病变；中药复方“糖 网清汤”能够在一定程度上减轻实验性糖尿病大鼠视网膜血管的病变，延缓病变的进 程，对早期糖尿病大鼠视网膜病变具有一定的防治作用。 主题词：糖尿病视网膜病变；中医药疗法；动物实验；糖网清汤。 Tang wang qing Decoction broth in diabetic rats and retinal blood flow change in rats Abstract Diabetes mellitus (DM) is a chronic metabolic disease that has been trended on global,with the economic development , the change of people’s lifestyle and aging population,the prevalence of type 2 Diabetes mellitus in worldside was increasing year by year ,especially in developing countries and some developed countries,the speed of increasa is faster. Objective:To investigate on the mechanism of the prevention and cure of DR by the TCM decoction named TWQD(Tang Wang Qing Decoction) through observing the The experimental streptozotocin (STZ) diabetic rat model caused, feeding 3 January, to the experimental rats fed with traditional Chinese medicine, Sugar Network broth and through the Blood Rheology, retinal blood vessels and The morphological changes of the retina to a preliminary study of traditional Chinese medicine, Sugar Network broth control mechanism of diabetic retinopathy. Methods: The streptozotocin (STZ)-induced diabetic rats were randomly divided into five groups: model group (M), calcium dobesilate group (A), sugar, low-dose group net broth (B), Sugar Network broth medium dose group (C), sugar, high-dose group net broth (D) (n = 10), and set the control group (