纳米雄黄对皮肤癌a431细胞株抑制增殖及抑制新生血管作用的实验研究.pdfVIP

Experimental Research of Realgar nanoparticles on cutaneous squamous Cell carcinoma in the Inhibition of proliferation and Neovascularization suppression Speciality: Clinical combination Medicine of TCM With Western Medicine Author: Lin Yanyan Tutor: Professor Li Xiurong Abstract Objective:Review the literature of TCM on skin tumor.Detect the cell proliferation and blood vessal growth regulatory factor expression of skin squamous cell carcinoma A431 cells treated with nanometer,then explore the realgar nanoparticles to anti-tumor mechanism of action. Methods:Skin squamous cell carcinoma A431 cells were cultured in vitro.Dose in the experimental groups and observe the changes in its morphology and growth conditions,respectively,using the MTT assay,immunohistochemistry and RT-PCR assay dosing inhibition of cell proliferation,the expression of pigment epithelium-derived factor(PEDF) and VEGFmRNA. Results:Realgar nanoparticles can inhibit human skin squamous cells carcinoma A431 cells proliferation and synergy with cisplatin.Realgar nanoparticles can ru-regulate the expression of PEDF and down the expression of VEGF to inhibit angiogenesis. Conclusion:In vitro experiments showed Realgar nanoparticles can significantly inhibit human skin squamous cell carcinoma A431 cells proliferation and treat cancer by inhibiting angiogenesis generate synergy with cisplatin. Key words Realgar nanoparticles; Skin squamous cell carcinoma A431 cells; inhibiting angiogenesis; Inhibition of proliferation 目 录 引 言 1 第一部分 实验研究 3 一 实验方法和结果 3 （一）一般材料与纳米雄黄的制备 3 （二）细胞培养 6 （三）纳米雄黄对皮肤鳞癌A431 细胞形态学和生长状