人急性髓系白血中医辨证分型与p15、id4基因甲基化的相关性研究.pdf

A stu d y o n th e C o rre la tio n b etw e en T C M sy n d ro m e d iffe r en tia tio n s a n d P 15 、 ID 4 g en e m eth y la tio n in h u m a n a cu te m y e lo id leu ke m ia S P ec ia l:In terna lm ed icin e o f T C M Stu d en t :L iu F ei Tu to r :P ro f X tl R tliro n g A b str a C t O bjectives:T o investigate the relation shiP b etw een T C M syndrom e diffe rentia一tion s an d th e P 15 、 ID 4 g en e P rom o ter m eth y latio n stattls in th e b o n e m arrow cells o f a cu te m yeloid leukem ia (A M L) Patients，and discu ss the relationshiP betw een P 15 、ID 4 gene m eth y latio n an d th e d ev e loP m en t o f d isea se ，to P rov id e a b a sis for clin ical d iag n o sis an d sy n drom e d iffe ren tiation o f acu te leu k em ia . M eth o d s : C o llected b o n e m arrow from 3 5 cases d iagn o sed in th e d iffe rent sub tyP es w ith d iffe ren t stag e of d isease of A M L P atien ts，th e P atien t accord in g to th e stan dard of th e d iagn o sin g tyP es of T C M d iv ided into : “deficiency of qiand yin，，(16 cases)，“Prevalence of toxic heat in the interior，，(6 cases)， “retention of Phlegm 一blood，，(13 cases)，another collection of 10 healthy bone m arrow to d o th e n orm a l con tro l，ex traction o f b o n e m arrow m on o n u clear cells. d etection m eth y lation stattls o f P 15 g en e 、 ID 4 g en e P ro m o ter w ith M eth y latio n sP ec ific P o ly m era se ch a in reaction (M S一P C 助 m ethod ，from the PersPective of m olecular b iology to discu ss the relation sh iP b etw een it an d T C M Sy n drom e一tyP e，an d statistically an aly zed th e resu lts. R esults: (l)P ositive rate ofP 15 and ID 4 gene m ethy lati