TRPV1在炎症性肠病大鼠模型结肠黏膜的表达及其与肥大细胞相关性研究(定稿)2011.02.10.docVIP

TRPV1在炎症性肠病大鼠结肠黏膜的表达及其与肥大细胞相关性研究 穆敬平，刘莉，周立志，敖金波, 程建明，廖恒 （湖北医药学院附属太和医院，湖北 十堰，442000） 基金项目：湖北省教育厅项目（ 通讯作者：刘莉（1978- ）,E-mail：liulimjp@163.com 摘要： 目的：探讨炎症性肠病IBD大鼠模型肠黏膜TRPV1表达和肥大细胞的变化, 以及二者在IBD 发病机制中的作用。方法：将16只大鼠随机分为正常组和模型组，建立炎症性肠病大鼠模型。采用RT-PCR检测两组大鼠结肠黏膜TRPV1的mRNA表达, 免疫组化方法检测TRPV1蛋白表达和肥大细胞数量。结果：与正常组相比, IBD组肠黏膜中TRPV1mRNA 和蛋白过度表达, IBD组肠黏膜肥大细胞数显著高于对照组( P0. 01)。正常对照组肠黏膜无或微弱阳性TRPV1表达。TRPV1蛋白及mRNA水平与肥大细胞数量之间呈正相关( r1= 0.528,r2=0.62，P0.01)。结论：IBD的发生发展与TRPV1的过度表达及肥大细胞数量变化密切相关。 关键词： 炎症性肠病；TRPV1；肥大细胞；相关性 Related research between TRPV1 and mast cell in inflammatory bowel disease. Mu Jing-ping, Liu Li, Zhou Li-zhi, Ao Jin-bo, Cheng Jian-ming,Liao Heng. Taihe Hospital affiliated to Hubei Medical College, Shiyan, Hubei province ，442000Fund Project: Project of Hubei Provincial Department of Education ( . Corresponding author: Liu Li (1978 -) . E-mail：liulimjp@163.com. Objective: To investigate the expression of TRPV1 expression and mast cell in IBD model，and the role in the pathogenesis of IBD. Methods: 16 rats were randomly divided into normal group and model group, established model of inflammatory bowel disease. TRPV1 mRNA of colon mucosa was examined by Semi-quantitative RT-PCR, TRPV1 protein expression and mast cells was examined by immunohistochemistry. Results: Compared with normal group, TRPV1mRNA and protein of IBD group was overexpressed, the number of intestinal mucosal mast cells of IBD group was significantly higher (P 0. 01). TRPV1 protein and mRNA levels and the number of mast cells was positively correlated ( r1= 0.528,r2=0.62，P0.01). Conclusion:Over-expression of TRPV1 involved in the development of IBD and had closely related mast cells. Key words: inflammatory bowel disease; TRPV1; mast cell; correlation 炎症性肠病(inflammatory bowel disease, IBD)是一类病因未明的肠道非特异性炎症, 具有慢性和消耗性的特征, IBD包括溃疡性结肠炎(ulcerative colitis, UC)和克罗恩病(Crohns disease, CD)IBD的发病机制尚未完全明确目前大多学者认为肠壁黏膜免疫调节异常、持续肠道感染、肠壁黏膜屏障缺损、遗传和环境等因素共同参与该疾病的发生发展[1-4]. 肠黏膜免疫调节细胞和多种细胞因子参与免