壮药千斤拔饮对卵巢大鼠免疫内分泌影响的研究.pdfVIP

目 录 中文摘要···················································2 英文摘要 ·····················································5 引 言 ·················································7 正 文 ·················································9 1.实验材料和方法·············································9 1.1 实验动物 ·················································9 1.1.1 实验药物及试剂········································10 1.2 方法 ·················································10 2. 实验结果·················································12 3.讨 论 ·················································16 4.存在的问题和展望 ···········································33 结 论 ·················································39 参考文献 ·················································36 附 录 ·················································40 综 述··················································48 致 谢 ·················································61 攻读学位期间发表的学术论文目录 ····························62 作者声明 ·················································63 个人简历 ·················································64 2 中 文 摘 要 目 的：从壮医理论出发，研究壮药千斤拔饮治疗围绝经期综合征的作 用机制。 方 法：本实验将3 月龄雌性SD 大鼠作为研究对象, 经切除双侧卵巢 造模成功后，受试大鼠随机分成4 组，每组 10 只，另取10 只3 月龄雌 性SD 大鼠为假手术对照组。A 组和E 组 （模型组及假手术组）,生理盐 水20ml/ kg.d-1 灌胃。B 组（千斤拔饮低剂量组）,予千斤拔饮膏剂11.7g/ kg 灌胃(为成人剂量的 10 倍)。C 组（千斤拔饮高剂量组）35.1g/ kg 灌胃， 为成人剂量的30 倍灌胃。D 组（西药对照组）, 己烯雌酚溶液0.05mg/kg.d-1 灌胃。以上动物每日灌胃1 次,连续给药4 周后，检测大鼠血清E 、FSH 、 2 LH 激素水平及IL - 2 的影响、剖取子宫称重、观察子宫组织学改变。 结 果：壮药千斤拔饮能够明显增加去卵巢大鼠的子宫重量指数，但子 宫内膜并无明显增厚；明显升高去卵巢大鼠血清 （E2 ）、IL-2水平，降低 去 （FSH ）和（LH-2 ）的水平，影响大鼠内分泌及免疫机能，与模型组 相比较,差异具有统计学意义(P 0. 05)，与假手术对照组比较, 差异无 统计学意义(P 0. 05)；本品在高剂量时，测定值与西药组比较，差异无 显著性意义（P 0. 05)，且高剂量组及西药组的测定值均与假手术组接 近。 结 论： 1.造模组结果显示，切除大鼠双侧卵巢可以成功建立围绝经期 模型。 2. 壮药千斤拔饮(高剂量组)能够明显升高去势大鼠血清E 含量，降低去