哈弗大学分子生物学课件-REPLICATIONB课件.pptVIP

* Looping the lagging strand to make both polymerases move in the same direction The discovery of DNA polymerase. Arthur Kornberg and Bob Lehman pursued an enzyme in bacterial extracts that would elongate a chain of deoxyribonucleic acid just like glycogen synthase elongates a chain of glycogen. The enzymatic activity was unusual: 1) Needed a template which dictates what nucleotide was added: substrate was directing enzymatic activity 2) Needed a primer annealed to the template. Wait a minute! John Cairns mutated the gene for DNA polymerase, polA, and the bacteria grew just fine! Eithe