EffectsofrecombinantbaculovirusAcMNPV教程分析.doc

Effects of recombinant baculovirus AcMNPV-BmKIT on the formation of early cables and nuclear polymerization of actin in Sf9 cells 重组杆状病毒AcMNPV-BmKIT对 Sf9细胞肌动蛋白早期电缆形成和的核聚合反应的作用 Abstract Autographa californica nuclearpoly hedrosis virus (AcMNPV) is one of the most important baculoviridae. However, the application of AcMNPV as a biocontrol agent has been limited. Previously, we engineered Buthus martensii Karsch insect toxin (BmK IT) gene into the genome of AcMNPV. The bioassay data indicated that the recombinant baculovirus AcMNPV-BmK IT signi?cantly enhanced the anti-insect ef?cacy of the virus. The actin cytoskeleton is the major component beneath the surface of eukaryotic cells. In this report, the effects of AcMNPV-BmK IT on the formation of early cables of actin and nuclear ?lamentous-actin (F-actin) were studied. The results indicated that these baculovirus induced rearrangement of the actin cytoskeleton of host cells during infection and actin might participate in the transportation of baculovirus from cytoplasm to the nuclei. AcMNPV-BmK IT delayed the formation of early cables of actin and nuclear F-actin and accelerated the clearance of actin in the nuclei. Introduction The actin cytoskeleton is the major component beneath the surface of eukaryotic cells. Many bacterial and viral pathogens induce rearrangement of the actin cytoskeleton of host cells during infection. This may be associated with viral genome replication, transcription, virus assembly, mobility, or budding (Cudmore et al. 1997). Several viruses have been shown to interact with the cytoskeleton or nuclear matrix in their replication, such varied activities as genome replication, protein synthesis and transport, and assembly, transport, and release of 摘要 苜蓿银蚊夜蛾多角体病毒(AcMNPV)是一种最重要的杆状病毒。 但是，将AcMNPV用作一种生物介质却很有限。 之前，我们建立了一种钳蝎属病毒(BmK IT)基因插入到AcMNPV基因组中。 活体鉴定数据表明杆状病毒重组体AcMNPV-BmK IT显著增强了昆虫的抗病毒性。 肌动蛋白是细胞骨架下层主要组分。 本文研究了AcMNPV-BmK IT对肌纤蛋白和核内肌动蛋白微丝早期电缆形成的作用。结果显示感染后，这些重组杆状病毒诱导了宿主细胞肌动蛋白骨架发生重排，而肌动蛋白可能参与转运杆状