新型维甲酸衍生物ATPR对肿瘤细胞株SKOV增殖及分化的影响.doc

新型维甲酸衍生物ATPR对肿瘤细胞株SKOV3增殖及分化的影响 吴繁荣，洪凡青，陈飞虎 （安徽医科大学药学院，安徽 合肥230032） [摘要] 目的：本研究探讨新型维甲酸衍生物4-氨基-2-三氟甲基苯基维甲酸酯（4-amino-2-trifluoromethyl-phenyl retinate，ATPR）对卵巢癌SKOV3细胞的增殖及分化作用。方法：ATPR作用于细胞株SKOV3后，MTT法检测细胞的增殖；吉姆萨染色法在倒置相差显微镜下观察加药处理前后细胞形态学变化；ELASA法检测卵巢癌标志物糖链抗原125(CA125)；流式细胞术检测细胞周期的变化情况。结果：ATP呈浓度依赖性抑制SKOV3细胞增殖，明显抑制作用出现在药物作用48h，但在72h后则更显著；倒置显微镜下观察发现细胞形态学趋于成熟分化；SKOV3中CA125水平下降；流式细胞仪测定G0/G1期细胞表达量增加，S期细胞表达量减少，细胞周期进程受影响，细胞阻滞在G0/G1期。结论：ATP对SKOV3细胞具有抑制增殖和一定的诱导分化作用。 关键词：ATPR；SKOV3细胞；增殖；诱导分化 作者简介：吴繁荣，男，硕士，讲师，研究方向：分子药理学，Tel：0551-5161115，E-mail：aydwfr@163.com 陈飞虎，通讯作者，男，教授，博士生导师，研究方向：分子药理学，Tel：0551-5161116，E-mail：cfhchina@ 本课题受国家“重大新药创制”科技重大专项(NO:2011ZX09401-021)、国家自然科学基金（NO资助 Effects of a new the retinoic acid derivatives ATPR on proliferation and differentiation of SKOV3 tumor cell WU Fan-rong，HONG Fan-qing， CHEN Fei-hu （School of Pharmacy，Anhui Medical University，Hefei 230032） [Abstract] Objective:To explore the effect of the 4-amino-2-trifluoromethyl-phenyl retinate (ATPR) on the proliferation and differentiation of SKOV3 tumor cell in vitro.Methods: Cell proliferation was assessed by MTT assay and growth curve of cells; Morphologic changes were observed after Giemsa staining using inverted phase contrast microscope; CA125 were measured by ELISA;The cell cycle were analyzed by Flow Cytometry( FCM).Results:The growth of SKOV3 cells was inhibited in a does-dependent manner after the treatment with ATPR.The phanero depressant effect appeared at 48 hours,but it is significanter after 72 hours;Cell morphous was observed to be mature in inverted microscope; The content of CA125 decreased; The proportion of cells in G0/G1 phase increased while the cells in S phase decreased.Cell cycle progression was blocked in the G0/G1 phase. Conclusion:ATPR could inhibit the proliferation and induce differentiation in some degree.The direction of differentiation is still need further study. Key words:ATPR;SKOV3 cell line;proliferation;induced differentiation  维甲酸( retinoic acid