非小细胞肺癌中miR-181a-5p功能的研究解析.docVIP

miR-181a-5p在非小细胞肺癌中的功能研究 [摘 要] 目的 探索miR-181a-5p 的抑癌机制，并探讨它作为标志物用于检测肺癌细胞的可行性。方法：采用Methods 1.To examine the cell growth and apoptosis, CCK8 and flow cytometry were applied ,respectively. 2. To determine the expression effects of miR-181a-5p, western blot blot was used.3. Transwell assay and wound healing assay. 4. Plasmid constructs and luciferase reporter assay.Results 1. miR-181a-5p inhibited cell proliferation and promoted cell apoptosis in vitro.2. Transwell assay and wound healing assay showed that miR-181-5p negatively regulated cell migration in vitro.3. miR-181a-5p down-regulated Kras e