环境工程毕业论文介绍.doc

南京林业大学 本科毕业设计（论文）? ?? 题 目：植物酯酶的提取及纯化研究 学 院： 化学工程学院 专 业： 环境工程 学 号： 070205223 学生姓名： 王康 指导教师： 范钦华 职 称: 讲师 二零一一 年 五 月 二十日 摘 要 本文通过实验条件筛选，初步确定了小麦酯酶的提取纯化方法有机磷农药活性抑制，结果如下： 1.通过单因素条件改变实验，确定了提取小麦粗酶液的固液比、温度、缓冲液pH值、提取时间、小麦粉目数的最优条件。 2.通过一次盐析及二次盐析，确定了通过盐析法初步提纯酶液的饱和度条件及提纯效果。 3.验证了超滤法在小麦酶液纯化过程中去除盐分的可行性及对酶液纯化的效果。 4.通过Sephadex G100凝胶层析后，顺利将酶液的比活力提高了7.2倍，确定了纯化酶液的方法。 5.通过绘制毒死蜱对小麦脂酶的活性抑制标准曲线，确定了小麦脂酶对于有机磷农药抑制作用的线性表现。 Abstract In this paper, the extraction and purification plants for lipase extraction and purification of the conditions, and the mapping of the organic phosphorus pesticide on wheat lipase inhibition standard curve, through literature review and selection of experimental conditions, initially identified wheat lipase Extraction and purification method and explore the inhibitory activity of organophosphorus pesticides for the preliminary nature of the experimental results are as follows: 1.Conditions by changing a single factor experiment, the crude enzyme extract of wheat solid-liquid ratio, temperature, buffer pH, extraction time, the best wheat flour mesh conditions. 2.And the second by a salting salting salting method determined by the initial saturation of purified enzyme solution conditions and the purification effect. 3.Validated ultrafiltration method in the wheat enzyme purification process to remove salt feasibility and effect of the purified enzyme. 4.By Sephadex G100 gel chromatography, the successful enzyme specific activity will be increased 7.2 times to determine the method of solution of pure enzyme. 5.By drawing on wheat of chlorpyrifos inhibited the activity of lipase standard curve to determine the organic phosphorus pesticide for wheat lipase inhibition of linear performance. 目 录 前言..............................................................................................................................