韭菜种质资源DNA指纹库构建及聚类分析.pdfVIP

河南农业科学,2011,40(8) : 164-168 Journal of Henan gricultural Sciences DNA * 刘宏敏, 张 明 , 李延龙, 曹秀敏, 王 贞, 乔保健, 尹守恒 ( , 467001) : 应用ISSR 分子标记方法, 以96份有代表性的韭菜资源为材料, 进行遗传多样性和亲缘 系分 析, 为韭菜品种创新提供理论依据结果表明, 从77条ISSR 引物筛选出20 条多态性好的引物,通过 PCR扩增P GE 指纹检测和凝胶成像指纹数据采集,建立第1 个韭菜种质资源DN 指纹库韭菜 DN 的ISSR 扩增多态性比率达94.7%, 是韭菜种质资源研究的一种有效分子标记应用Ne-i li 相 似系数法估算了96 份材料间的遗传相似系数(genetic similarity,Gs) , 其GS 在0.58~ 0.88,遗传多样 性比较丰富部分材料聚类与其来源地有明显相 性, 与其亲缘 系较近有 , 符合植物种群分布一 般规律;新疆野生韭等个别品种自成 一类, 与其特异的遗传基础有 ;选育推广的韭菜品种聚类比较 集中,遗传基础相对狭窄,所以在新品种创新研究中要加强国内外远缘韭菜种质的导入 : 韭菜; 种质资源; DN 指纹库; 构建; 聚类分析 : S633.3 : : 1004-3268(2011)08-0164-05 DN Fingerprint Library Construction and Cluster nalysis of Leek Germplasm Resources * LIU Hong-min,ZH NG Ming ,LI Yan-long, C O Xiu-min,W NG Zhen, QI O Bao-jian, YIN Shou-heng ( Pingdingshan cademy of gricultural Sciences,Pingdingshan 467001, China) Abstract: In order to provide theoretical information for leek breeding, this study was to probe genetic diversity of the leek germplasms. 96 leek samples were used to detect the intra-and inter- specific geneticvariation by inter-simple sequence repeat (ISSR) analysis. The result showed that 20 ISSR primers were screened from 77 primers based on 96 leek samples. T he first leek DN fingerprint library was constructed by PCR amplification, P GE fingerprint detection, and fin- gerprint data collection of Gel imaging. The ISSR amplification diversity reached 94.7%, which was an effective molecular marker. The genetic similarity (GS) among 96 leek samples ranged from 0.58 to 0. 88, which could fully show that they have rich genetic diversity. The result of cluster analysis showed that the cl