西芹腐根浸提液处理后黄瓜枯萎病菌致弱机制.pdfVIP

科技导报2014，32（14） 西芹腐根浸提液处理后黄瓜枯萎病菌西芹腐根浸提液处理后黄瓜枯萎病菌 致弱机制致弱机制 李杰，云兴福，高晓敏，王勇，钱程，马立国 内蒙古农业大学农学院，呼和浩特010019 摘要摘要 室内条件下用西芹腐根乙醇、丙酮和蒸馏水浸提液，连续5代处理黄瓜枯萎病菌，测定处理后黄瓜枯萎病菌分泌的镰刀菌 酸量和细胞壁降解酶活性及其与致病力的相关关系，以揭示西芹腐根浸提液连续多代处理黄瓜枯萎病菌的致弱机制。结果表 明，各处理的致病力随接种代数的增加逐渐减小，镰刀菌酸质量浓度和果胶酶活性随培养代数的增加逐代减少，纤维素酶活性随 培养代数的增加无明显的变化规律，β-葡萄糖苷酶活性随培养代数的增加逐代升高，但均表现为处理低于对照。相关分析表明， 致病力与菌体分泌的镰刀菌酸质量浓度和果胶酶活性均表现为显著或极显著的正相关关系，与纤维素酶活性相关性不显著；与 培养第3~5代β-葡萄糖苷酶活性表现为显著或极显著的正相关关系。 关键词关键词 西芹；腐根浸提液；镰刀菌酸；细胞壁降解酶 中图分类号中图分类号 S436.421.1 文献标志码文献标志码 A doidoi 10.3981/j.issn.1000-7857.2014.14.003 AttenuationMechanismofParsleyRottenRootExtracton Fusariumoxysporum f.sp.cucumerinum LIJie,YUNXingfu,GAOXiaomin,WANGYong,QIANCheng,MALiguo CollegeofAgriculture,InnerMongoliaAgriculturalUniversity,Hohhot 010019,China AbstractAbstract Thispaperinvestigatestheattenuationmechanismofparsleyrottenrootextracton Fusariumoxysporum f.sp. cucumerinum (Foc.).Foc.wastreatedfromthefirsttofifthgenerationwithparsleyrottenrootextractpreparedbyethanol,acetone,anddistilled water. The excretive fusarium acid content and cell wall degradation enzyme activity were detected and their correlation with pathogenicitywasstudied.Theresultsshowthatthepathogensfusariumacidcontentandpectinaseenzymeactivityweresignificantly reducedwiththeincreaseoftreatedgenerations,thecelluloseenzymeactivityhadnoapparentchangewiththeincreaseoftreated generations,andtheβ-glycosidaseenzymeactivityincreasedwiththeincreaseoftreatedgenerations,butallwerelowerthanthosein the control group. Correlation analysis shows that there are significant or extremely significant positive correlation between pathogenicityandthepathogensfusariumacidcontent,pectinaseenzymeactivity,andβ-glycosidaseenzymeactivity,butthereisno clearrelationshipbetweenpathogenicityandthecelluloseenzymeactivity. KeywordsKeywords parsley;rottenrootextracts;fusaricacid;cellwalldegradationenzyme 黄瓜枯萎病（Fusariumoxyspor