动力车间班组会议签到表课程.docVIP

动力车间班组会议签到表 序号 姓 名 2009年 月 日 1 胡? 平 2 汪圣奎 3 杜? 波 4 冯会平 5 李? 兵 6 张彩华 7 庄玉萍 8 浦明红 9 王? 华 10 高? 艳 11 藏玉成 12 李? 波 动力车间班组会议签到表 序号 姓 名 2009年 月 日 1 饶四新 2 闫雪兵 3 胡云涛 4 熊明串 5 杨? 宏 6 周? 易 7 胡? 均 8 史臻峰 9 望菊红 10 童秋莹 11 朱应新 12 席燕燕 动力车间班组会议签到表 序号 姓 名 2009年 月 日 1 夏世新 2 刘应兵 3 李? 勇 4 陈金凤 5 龚? 勇 6 庄爱华 7 刘? 莉 8 杜红艳 9 张? 健 10 李? 华 11 黄兆兰 12 詹媛媛 动力车间班组会议签到表 序号 姓 名 2009年 月 日 1 张? 坤 2 田国良 3 黄小平 4 钱? 密 5 倪陈蓉 6 张勤武 7 李? 丽 8 梅? 艳 9 周小琴 10 甘红莲 11 徐卫华 12 罗 春 Acknowledgments The authors would like to thank Johns Hopkins University for the TC-1 cells. This work was supported by a National Health Research Institutes intramural grant (IV-103-PP-22) and grants from the National Science Council, which were awarded to Y.C. Song (NSC 99-2321-B-400-004-MY3) and S.J. Liu (NSC 103-2321-B-400-008). Author Contributions Y.C.S. and S.J.L. designed the studies. Y.C.S. performed the research and analyzed the data. Y.C.S. and S.J.L. wrote the manuscript. Additional Information C57BL/6 mice were immunized subcutaneously (s.c.) once with 1 μ g of peptide mixed with or without 10 μ g of CpG adjuvant. After one week, splenocytes were harvested, and the response of IFN-γ -secreting cells was determined by ELISPOT after 48 h of peptide stimulation. Briefly, 2 × 105 splenocytes were incubated with 1 μ g/ml irrelevant peptide or RAH peptide in an anti-IFN-γ -coated polyvinylidene fluoride (PVDF) plate for 48 h. After incubation, the cells were removed, and a biotinylated anti-IFN-γ Ab (eBioscience, San Diego, CA, USA) was added to each well. The plates were incubated at 37 °C for 2 h. Following the addition of the avidin-HRP reagent (eBioscience, CA, USA), the assay was developed using a 3-amine-9-ethyl carbazole (AEC; Sigma-Aldrich, M