实验九用牛顿环干涉测量透镜曲率半径实验报告课程.docVIP

班级_____________学号____________姓名___________带课老师____________完成实验时间_______________签名序号_____________预习检查____________实验九 用牛顿环干涉测透镜曲率半径实验目的1.掌握用牛顿环测定透镜曲率半径的方法;2.通过实验加深对等厚干涉原理的理解;实验仪器和用具钠灯,牛顿环仪,玻璃片(连支架),移测显微镜实验原理详见教材P113-116.实验内容1 实验数据处理表1 牛顿环相应级数暗环位置读数(零点读数:0.004)单位:cm 1 21.592 27.690 21.898 27.364 22.257 27.008 22.684 26.570 23.251 26.028 2 27.475 21.362 27.170 21.676 26.802 22.052 26.371 22.476 25.808 23.052 3 21.579 27.689 21.893 27.365 22.261 27.009 22.682 26.567 23.249 26.021 表2 牛顿环相应级数暗环半径(零点读数:0.004)单位:cm 1 6.098 5.466 4.751 3.886 2.777 2 6.113 5.494 4.750 3.895 2.756 3 6.110 5.472 4.748 3.885 2.772 平均值6.107 5.477 4.750 3.889 2.768 透镜曲率半径 注:,,,;;. 2 标准不确定度的分析(1)牛顿干涉圆环暗环半径r的标准不确定度的A类不确定度:; 的B类不确定度:(均匀分布取); 的总不确定度:;同理可得:;;;(2)透镜曲率半径R的不确定度透镜曲率半径R的标准不确定度为.采用高斯误差传播方法简化公式可得: 由;可得: 故:;; ; ; 故,R的总不确定度为:; 所以,透镜的曲率半径为:.实验结论实验的误差主要来源于,暗纹有一定的宽度,特别是级数较低的圆环其宽度较大,故难以精确的测得暗环的半径.要尽量减少该测量的误差,可尽可能的测级数较大的暗环,但要注意条纹间距较小而清晰度会较低.Acknowledgments The authors would like to thank Johns Hopkins University for the TC-1 cells. This work was supported by a National Health Research Institutes intramural grant (IV-103-PP-22) and grants from the National Science Council, which were awarded to Y.C. Song (NSC 99-2321-B-400-004-MY3) and S.J. Liu (NSC 103-2321-B-400-008). Author Contributions Y.C.S. and S.J.L. designed the studies. Y.C.S. performed the research and analyzed the data. Y.C.S. and S.J.L. wrote the manuscript. Additional Information C57BL/6 mice were immunized subcutaneously (s.c.) once with 1 μ g of peptide mixed with or without 10 μ g of CpG adjuvant. After one week, splenocytes were harvested, and the response of IFN-γ -secreting cells was determined by ELISPOT after 48 h of peptide stimulation. Briefly, 2 × 105 splenocytes were incubated with 1 μ g/ml irrelevant peptide or RAH peptide in an anti-IFN-γ -coated polyvinylidene fluoride (PVDF) plate for 48 h. After incubation, the cells were removed, and a biotinylated anti-IFN-γ Ab (eBioscien