锻件毛坯计算课程.docVIP

锻件毛坯计算 如图，根据零件图绘制锻件图。 在1吨模锻锤上模锻，生产批量为成批生产。 解：计算过程如下 1、确定机械加工余量和公差 （1）计算锻件质量m （2）计算锻件复杂系数S S在范围内，所以复杂系数为级。 （3）材质系数M 45钢含碳量c%=0.42~0.50%0.65% 所以材质系数为级。 （4）由表2-2、表2-3查得零件加工余量 锻件厚度尺寸100mm，余量2.0~2.5mm，取2.5mm。 锻件长度尺寸132mm，余量2.0~2.5mm，取2.5mm。 内孔直径尺寸46mm，余量2.6mm。 （5）根据锻件质量m、复杂系数S、材质系数M，由表2-4、2-6查公差 锻件尺寸132mm，公差； 锻件尺寸90mm，公差； 厚度尺寸100mm，公差； 厚度尺寸36mm，公差； 内孔直径46mm，公差； 错差1.2mm； 残留飞边公差1.2mm； 表面缺陷，不允许超过1.2mm。 2、确定模锻斜度 由零件尺寸，查得，内表面拔模斜度，外表面拔模斜度。 3、圆角半径 外圆角半径r=余量+a=2.5+2=4.5mm，取r=5mm。 内圆角半径R=（2~3）r，根据需要，取R=8mm。 4、冲孔连皮 连皮厚度 取 圆角半径 综上得，锻件图如下。 Acknowledgments The authors would like to thank Johns Hopkins University for the TC-1 cells. This work was supported by a National Health Research Institutes intramural grant (IV-103-PP-22) and grants from the National Science Council, which were awarded to Y.C. Song (NSC 99-2321-B-400-004-MY3) and S.J. Liu (NSC 103-2321-B-400-008). Author Contributions Y.C.S. and S.J.L. designed the studies. Y.C.S. performed the research and analyzed the data. Y.C.S. and S.J.L. wrote the manuscript. Additional Information C57BL/6 mice were immunized subcutaneously (s.c.) once with 1 μ g of peptide mixed with or without 10 μ g of CpG adjuvant. After one week, splenocytes were harvested, and the response of IFN-γ -secreting cells was determined by ELISPOT after 48 h of peptide stimulation. Briefly, 2 × 105 splenocytes were incubated with 1 μ g/ml irrelevant peptide or RAH peptide in an anti-IFN-γ -coated polyvinylidene fluoride (PVDF) plate for 48 h. After incubation, the cells were removed, and a biotinylated anti-IFN-γ Ab (eBioscience, San Diego, CA, USA) was added to each well. The plates were incubated at 37 °C for 2 h. Following the addition of the avidin-HRP reagent (eBioscience, CA, USA), the assay was developed using a 3-amine-9-ethyl carbazole (AEC; Sigma-Aldrich, MO, USA) staining solution. The reaction was stopped after 4–6 min by placing the plate under tap water. The spots were counted using an ELISP