一个螺栓的画法课程.docVIP

一个画螺栓螺母三维的教程 2006年12月05日 星期二 上午 09:35 一个画螺栓螺母三维的教程第一步:加载THREAD.LSP(论坛有下栽)选俯视图L:画一条高25中心线命令:Metric公称外径:12(画12mm螺纹)螺距:1.75(基本螺牙)起始点:(中心线底端)螺纹总长(Y方向):25会生成这样的图: 第二步:转西南等轴侧视图移动原点在圆心上定义新的UCS命令:ucs以圆心点为圆心画直径10.25圆,然后拉伸20 命令:extrude 第三步:再移动原点,在拉伸轴的另一面圆心上定义新的UCS命令:ucs以原点画正六边形外切于直径19的圆拉伸(命令:extrude)9 第四步:着上色基本好了,下面来做倒角拉伸中心线到六角顶面回到主视图用三维动态观察转动到一定位置作六角中心到任一角的连接线垂直画一小三角 第五步:把多余的线擦掉,“小三角”转换为面域(绘图—面域(必须在一个平面,闭合)),沿中心线旋转(命令“revolve) 第六步:差集(命令:)后一个三维螺栓就好了 第七步;在螺栓的基础上画螺母就更方便了打开左视图在正交摸式下把六角形螺头向左移动35差集后一个三维螺母也就好了 Acknowledgments The authors would like to thank Johns Hopkins University for the TC-1 cells. This work was supported by a National Health Research Institutes intramural grant (IV-103-PP-22) and grants from the National Science Council, which were awarded to Y.C. Song (NSC 99-2321-B-400-004-MY3) and S.J. Liu (NSC 103-2321-B-400-008). Author Contributions Y.C.S. and S.J.L. designed the studies. Y.C.S. performed the research and analyzed the data. Y.C.S. and S.J.L. wrote the manuscript. Additional Information C57BL/6 mice were immunized subcutaneously (s.c.) once with 1 μ g of peptide mixed with or without 10 μ g of CpG adjuvant. After one week, splenocytes were harvested, and the response of IFN-γ -secreting cells was determined by ELISPOT after 48 h of peptide stimulation. Briefly, 2 × 105 splenocytes were incubated with 1 μ g/ml irrelevant peptide or RAH peptide in an anti-IFN-γ -coated polyvinylidene fluoride (PVDF) plate for 48 h. After incubation, the cells were removed, and a biotinylated anti-IFN-γ Ab (eBioscience, San Diego, CA, USA) was added to each well. The plates were incubated at 37 °C for 2 h. Following the addition of the avidin-HRP reagent (eBioscience, CA, USA), the assay was developed using a 3-amine-9-ethyl carbazole (AEC; Sigma-Aldrich, MO, USA) staining solution. The reaction was stopped after 4–6 min by placing the plate under tap water. The spots were counted using an ELISPOT reader (Cellular Technology Ltd., Shaker Hei