CAD螺栓、螺纹画法实例演示课程.docVIP

CAD螺栓?螺纹画法实例演示 粗牙螺栓M16图形实例 画法步骤 画法的总思路:六角头实体,光杆部分实体,螺纹部分实体?此处只介绍螺纹部分实体? 查标准计算出M16粗牙螺纹相关数据?三角形牙型数据,以12.75直径划螺旋,命令:,比如划4圈螺纹,圈高以螺距2为基准,但是在实际确定圈高时要略大于螺距,如2.01,为了在扫掠时能够实现,如果等于螺距牙型不能扫掠,所以只能略大于螺距的近似画法,各种螺纹近似圈高实际尺寸可以自己总结? 画出原始牙型,方向如下图 扫掠牙型,选择基点选项扫掠,路径旋转螺旋线,以图中边中点为基点?实现以下实体? 以上螺纹已经画出,下面修正牙型: 在螺纹中心画直径13.84圆柱实体,修正原始小径为螺纹小径取并集? 画内直径16,外直径超过螺纹计算大径大一些的圆筒,差集以修正螺纹大径? 至此螺纹部分完成?把此部分存入块以备插入使用,长度方向复制后并集可以得到不同长度的螺纹长度? Acknowledgments The authors would like to thank Johns Hopkins University for the TC-1 cells. This work was supported by a National Health Research Institutes intramural grant (IV-103-PP-22) and grants from the National Science Council, which were awarded to Y.C. Song (NSC 99-2321-B-400-004-MY3) and S.J. Liu (NSC 103-2321-B-400-008). Author Contributions Y.C.S. and S.J.L. designed the studies. Y.C.S. performed the research and analyzed the data. Y.C.S. and S.J.L. wrote the manuscript. Additional Information C57BL/6 mice were immunized subcutaneously (s.c.) once with 1 μ g of peptide mixed with or without 10 μ g of CpG adjuvant. After one week, splenocytes were harvested, and the response of IFN-γ -secreting cells was determined by ELISPOT after 48 h of peptide stimulation. Briefly, 2 × 105 splenocytes were incubated with 1 μ g/ml irrelevant peptide or RAH peptide in an anti-IFN-γ -coated polyvinylidene fluoride (PVDF) plate for 48 h. After incubation, the cells were removed, and a biotinylated anti-IFN-γ Ab (eBioscience, San Diego, CA, USA) was added to each well. The plates were incubated at 37 °C for 2 h. Following the addition of the avidin-HRP reagent (eBioscience, CA, USA), the assay was developed using a 3-amine-9-ethyl carbazole (AEC; Sigma-Aldrich, MO, USA) staining solution. The reaction was stopped after 4–6 min by placing the plate under tap water. The spots were counted using an ELISPOT reader (Cellular Technology Ltd., Shaker Heights, OH, USA). For RAH-specific T cell staining, spleens were harvested seven days