6152-155j167工艺技术4-10植物乳杆菌代谢产细菌素培养基优化.pdfVIP

现代食品科技 Modern Food Science and Technology 2012, Vol.28, No.2 植物乳杆菌代谢产细菌素的培养基优化 1 2 2 2 2 佟世生 ，解洛香，徐乐，胡涛，刘萍 （1.北京城市学院生物医药学部,北京 100083 ）（2.中国农业大学食品科学与营养工程学院,北京 100083 ） 摘要：本文对植物乳杆菌代谢产细菌素的培养基进行一系列优化。结果显示，不同培养基对菌株生长和细菌素产量有重 要的影响，其中以碳源的影响最为显著。综合考虑，5%糖蜜、0.5%酵母膏、2%胰蛋白胨、0.4% KH PO 、0.1% 2 4 MgSO ·7H O 、0.5% CaCO 、0.05% MnSO 和0.3%吐温80是植物乳杆菌生长和代谢产细菌素的最优培养基组合。 4 2 3 4 关键词：植物乳杆菌；细菌素；培养基优化 文章篇号：1673-9078(2012)2-152-155 Culture Medium Optimization of Lactobacillus Plantarum for Bacteriocin Production 1 2 2 2 2 TONG Shi-sheng , XIE Luo-xiang , XU Le , HU Tao , LIU Ping (1.College of Biological Medicine, Beijing City University, Beijing 100083, China) (2.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) Abstract: In this paper, the culture medium of Lactobacillus plantarum for bacteriocin production was optimized. The results showed that the culture medium had an important effect on germ formation and production of bacteriocin, in which carbon source was significant. Overall, 5% molasses, 0.5% yeast extract, 2% tryptone, 0.4% KH PO , 0.1% MgSO ·7H O, 0.5% CaCO , 0.05% MnSO and 2 4 4 2 3 4 0.3% Tween 80 were the optimal combination of culture medium. Key words: Lactobacillus plantarum ; bacteriocin; cultu