反相高效液相色谱法测定蛹虫草中腺苷与虫草素的含量.pdfVIP

172 2010, Vol. 31, No. 16 食品科学 ※分析检测 反相高效液相色谱法测定蛹虫草中腺苷和 虫草素的含量 刘小芳，薛长湖* ，常耀光 (中国海洋大学食品科学与工程学院，山东青岛 266003) 摘 要：建立一种新的反相高效液相色谱方法，用于蛹虫草中腺苷和虫草素的定性定量分析。样品经水超声提取， 以终浓度20mmol/L 柠檬酸、40mmol/L 醋酸、20mmol/L 三乙胺的混合溶液- 乙腈(体积比98:2)为流动相，流速1.0mL/ min ，色谱柱Cap cellpak C18( 150mm ×4 .6mm ，5 μm)分离，柱温40 ℃，进样量10 μL ，紫外检测波长260nm 。在 2 此条件下，腺苷在1～1000 μg/mL 范围内有良好的线性关系(Y=33574X +54.526 ，R =0.9999) ，检测限0.2 μg/mL ，回 收率93 .75% ～96 .90% ，RSD 为0. 116% ；虫草素在2 ～1000 μg/mL 范围内有良好的线性关系( Y=343 85X +23 .103 ， 2 R = 1.0000) ，检测限0 .2 μg/m L ，回收率92 .03 % ～97 .35% ，R SD 为0 . 104 % 。该方法样品处理简便、分离度高、 杂质干扰小、回收率高、重复性好，能够对蛹虫草中腺苷和虫草素进行准确的定性定量分析。 关键词：反相高效液相色谱法；蛹虫草；腺苷；虫草素 Analysis of Adenosine and Cordycepin in Cordy cep s militaris (L.) Link by Reversed Phase-High Performance Liquid Chromatography LIU Xiao-fang ，XUE Chang-hu * ，CHANG Yao-guang (College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China) Abstract ：In this paper, a RP-HPLC method for determining the contents of adenosine and cordycepin in Cordy cep s militaris ( L.) Link is described. The separation column was Capcellpak C18 (150 mm ×4.6 mm, 5 μm), which was held at 40 ℃. The mobile phase was composed of a mixed solution containing 20 mmol/L citric acid, 40 mmol/L acetic acid and 20 mmol/L triethylamine and acetonitrile (V/ V, 98:2) at a flow rate of 1.0 mL/min. The sample loading volume was 10 μL. UV detection was performed at 260 nm. The method showed a good linear relationship between peak area and concentration over the range from 1